Chromatographic separation column used under negative pressure condition

Abstract

A chromatographic separation column used under a negative pressure condition comprises a glass tube; an upper tube joint, a vacuum plug, an upper vacuum screw cap, and an upper vacuum nut which are disposed at one end of the glass tube from top to bottom; the upper tube joint is sealedly connected with the vacuum plug; the vacuum plug contacts an upper vacuum screw cap through a sheath surface; a sealing ring is disposed between the upper vacuum screw cap and the upper vacuum nut; the glass tube is sleeved with the sealing ring; a lower vacuum nut, a lower vacuum screw cap, and a lower tube joint are disposed at the other end of the glass tube from top to bottom; the lower vacuum screw cap is sealedly connected with the lower tube joint; a sealing ring is disposed between the lower vacuum screw cap and the lower vacuum nut; the sealing ring is disposed on the glass tube; a sieve plate is disposed in the lower vacuum screw cap. The invention has a reasonable structure, is convenient for application, has relatively low requirements for chromatographic separation column sealing materials and mechanical processing precision, has greatly reduced cost, and is an ideal chromatographic separation column used under a negative pressure condition.

Description

technical field [0001] This application relates to a negative pressure chromatographic separation column for chromatographic separation instruments, which can achieve the separation of target objects under negative pressure conditions. The chromatographic separation column is mainly used for food analysis, environmental analysis and drug metabolism, drug analysis, and biological detection , drugs and stimulants and other analysis and testing process. Background technique [0002] With the development of modern chromatographic analysis technology, the detection of target substances in drugs, environmental and biological samples has begun to develop towards trace and ultra-trace, so chromatographic analysis has put forward higher and higher requirements for sample pretreatment. At present, the instruments that can separate the target components in the sample mainly include high performance liquid chromatography (HPLC), gel chromatography (GPC), column chromatography and solid ...

AI Technical Summary

Problems solved by technology

[0002] With the development of modern chromatographic analysis technology, the detection of target substances in drugs, environmental and biological samples has begun to develop towards trace and ultra-trace, so chromatographic analysis has put forward higher and higher requirements for sample pretreatment. At present, the instruments that can separate the target components in the sample mainly include high performance liquid chromatography (HPLC), gel chromatography (GPC), column chromatography and solid phase extraction, among which high performance liquid chromatography and gel chromatography The chromatographic separation column used in the chromatographic instrument is a chromatographic separation column in a high-pressure environment. It has extremely strict requirements on the sealing performance of the chromatographic separation column structure, including the material, seals and machining accuracy of the chromatographic separation column. Therefore, this type of chromatographic separation The cost of the column is high, and the chromatographic separation column of the column chromatograph is usually used under negative pressure conditions, and there is no negative pressure chromatographic separation column suitable for automation

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