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Method by utilizing double markers to acquire share of inorganic carbon source utilized by plants

An inorganic carbon source and double-labeling technology, applied in the field of ecological environment management, can solve the problems of large measurement errors, cumbersome steps, and many measurement indicators, and achieve the effect of reliable data, simple calculation, and fewer steps

Active Publication Date: 2012-06-27
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

[0023] The technical problem to be solved by the present invention is to provide a method for obtaining the share of inorganic carbon sources used by plants by using double labels, so as to overcome the problem of many measurement indicators and short measurement periods in the prior art due to the fact that the values ​​of the two ends come from different experiments. Long, cumbersome steps and large measurement errors

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  • Method by utilizing double markers to acquire share of inorganic carbon source utilized by plants

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Embodiment Construction

[0032] Embodiments of the present invention: it comprises the following steps, the first, measure the sodium bicarbonate produced by different producers, select two kinds of δ 13Sodium bicarbonate with a C value difference greater than 8 ‰ was added to the nutrient solution as isotope label 1 culture solution and isotope label 2 culture solution to cultivate plants. Other culture conditions are the conditions to be investigated, such as pH, and the proportion of inorganic carbon sources utilized by the plant can be selected under which pH value needs to be investigated. Isotope labeling δ of bicarbonate ion in culture medium 13 C value is δ C1 , δ of bicarbonate ion in isotope-labeled 2 culture solution 13 C value is δ C2 ,δ C1 and δ C2 The difference is generally greater than 8 ‰ (PDB). Second, after culturing until there are more than 4 true leaves, measure the stable carbon isotope composition δ of the corresponding leaves of the investigated plants cultured in the tw...

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Abstract

The invention discloses a method by utilizing double markers to acquire share of inorganic carbon source utilized by plants, which comprises the following steps that: A. sodium bicarbonate produced by different manufacturers is determined, two types of sodium bicarbonate with delta 13C difference being more than eight thousandths are used as an isotopic marker 1 and an isotopic marker 2 to be respectively added into nutrient fluid to culture plants; delta 13C value of bicarbonate ion in nutrient fluid of the isotopic marker 1 is delta C1, and delta 13C value of bicarbonate ion in nutrient fluid of the isotopic marker 2 is delta C2; and B. after more than four leaves appear, the values of delta 13C consisting of stable carbon isotopes of plant leaves to be observed correspondently culturedby the nutrient fluid of the two types of isotopic markers are respectively determined as delta T1 and delta T2; and C. delta C1, delta C2, delta T1 and delta T2 are brought into equation fB= to calculate the share fB of the carbonate ions utilized by the plants. Due to the adoption of the method, the share of the inorganic carbon source utilized by the plants can be rapidly acquired, the steps are fewer, simplicity in calculation can be realized, and data is reliable.

Description

technical field [0001] The invention relates to a method for obtaining the share of inorganic carbon sources used by plants by using stable carbon isotopes, and belongs to the field of ecological environment management. Background technique [0002] Plants can not only use carbon dioxide in the air as a raw material for photosynthesis, but also use bicarbonate ions stored in leaves as a raw material for photosynthesis. The proportion of plants using different inorganic carbon sources has a significant relationship with the plant's karst fitness, and plays an important role in screening karst suitable plants and using biological methods to control and restore the fragile karst ecological environment. [0003] The strong fractionation signature of stable carbon isotopes is the basis for identifying sources of inorganic carbon in plant matter. There are two stable isotopes of carbon in nature: 12 C and 13 C, their natural average abundances are 98.89% and 1.11%, respectively...

Claims

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Application Information

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IPC IPC(8): A01G31/00
Inventor 吴沿友邢德科李海涛刘莹
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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