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Method for producing crocin with higher than 95% purity from gardenia

A technology of saffron and gardenia, which is applied in the direction of chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems that are difficult to realize industrial production and affect the application of saffron active substances, achieve low cost and improve column efficiency , The effect of simple process

Active Publication Date: 2014-04-09
QIANJIANG GREEN SEA TREASURE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology is difficult to realize industrial production, which directly affects the application of the active substance saffron

Method used

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  • Method for producing crocin with higher than 95% purity from gardenia
  • Method for producing crocin with higher than 95% purity from gardenia
  • Method for producing crocin with higher than 95% purity from gardenia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Extraction: crush 1 kg of dried gardenia fruits to 10-40 mesh, soak in 8-15 kg of pure water at room temperature for 1 hour, then percolate at an appropriate flow rate, share 25-35 kg of pure water for extraction, 4 After 8 hours of percolation and extraction, extract A was obtained.

[0023] 2. Microfiltration: Pass the extract A through a 0.2-1 micron ceramic membrane to obtain a clear filtrate B.

[0024] 3. Sample adsorption: use 3 sections of ZT-02 macroporous adsorption resin column, each section of 70ml, the clarified filtrate B is first adsorbed on the first section of the column, when the effluent is reddish, in the first section of the column Connect the second column to the end, and continue to adsorb until the effluent of the second column is reddish, string the third column C at the end of the second column, until the effluent of column C is reddish, remove column C . Re-attach column D to the end of the second section of the column, follow the same method ...

Embodiment 2

[0029] 1. Extraction: crush 10 kg of dried gardenia fruits to 10-40 mesh, soak in 80-150 kg of pure water at room temperature for 1 hour, then percolate at an appropriate flow rate, share 250-350 kg of pure water for extraction, 4 After 8 hours of percolation and extraction, extract A was obtained.

[0030] 2. Microfiltration: Pass the extract A through a 0.2-1 micron ceramic membrane to obtain a clear filtrate B.

[0031] 3. Sample adsorption: use 3 sections of ZT-02 macroporous adsorption resin column, each section of 1000ml, the clarified filtrate B is first adsorbed on the first section of the column, until the effluent is reddish, in the first section of the column Connect the second column to the end, and continue to adsorb until the effluent of the second column is reddish, string the third column C at the end of the second column, until the effluent of column C is reddish, remove column C . Re-attach column D to the end of the second section of the column, follow the same...

Embodiment 3

[0036] 1. Extraction: crush 100 kg of dried gardenia fruits to 10-40 mesh, soak in 800-1500 kg of pure water at room temperature for 1 hour, then percolate at an appropriate flow rate, share 2500-3500 kg of pure water for extraction, 4 After 8 hours of percolation and extraction, extract A was obtained.

[0037] 2. Microfiltration: Pass the extract A through a 0.2-1 micron ceramic membrane to obtain a clear filtrate B.

[0038] 3. Sample adsorption: use 3 sections of ZT-02 macroporous adsorption resin column, each section of 10 liters, the clarified filtrate B is first adsorbed on the first section of the column, until the effluent is reddish, in the first section of the column Connect the second column to the end, and continue to adsorb until the effluent of the second column is reddish, string the third column C at the end of the second column, until the effluent of column C is reddish, remove column C . Re-attach column D to the end of the second section of the column, follow ...

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Abstract

The invention relates to a method for producing crocin with higher than 95% purity from gardenia. The invention adopts a normal-temperature water-percolation extraction process, can effectively reduce the extraction of pectins, proteins and other impurities, and avoids difficulty in the subsequent separation and purification. Before extraction, seed crushing is avoided in the gardenia fruit pulverizing process, thereby reducing the extraction of oil. Water extract microfiltration can remove solid particles, pectins and other impurities in the water extract and filter out bacteria and other microbes, and the microfiltered water extract can be directly continuously adsorbed by a macroporous resin column. Fine macroporous resin is used instead of conventional large-particle-diameter macroporous resin for adsorption chromatography, normal pressure technology is upgraded to pressurization technology, and a continuous circulation chromatography method is used instead of the traditional linear chromatography method, thereby enhancing the column efficiency and chromatography efficiency; and in cooperation with the refinement of the C18 column, the pure crocin can be directly prepared. The whole technological process is simple, has the advantages of solvent saving, low cost, high efficiency and environmental protection, and is suitable for industrial production of the crocin product of which the purity is higher than 95%.

Description

Technical field [0001] The invention relates to a method for producing crocin with a purity greater than 95% using gardenia as a raw material, and belongs to the field of extraction, separation and purification of natural products. Background technique [0002] The mature fruit of gardenia contains about 0.5% of crocin, also known as α-crocin and crocin-1, which are water-soluble carotenoid compounds with strong free radical scavenging, antioxidant and anti-cancer activities. The preparation of pure crocin is difficult, even if the purity of the standard product purchased from Sigma is less than 95%. At present, the laboratory extraction of crocin is usually leached with water or water-containing ethanol, followed by repeated chromatography and gradient elution through a macroporous adsorption resin column and a silica gel column, and then through recrystallization to obtain pure crocin. The existing technology is difficult to realize industrialized production, which directly af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/06C07H1/08
Inventor 袁晓王国艳叶巍周东斌林顺权杨威曹健林志芬林立克杨毅融
Owner QIANJIANG GREEN SEA TREASURE BIO TECH
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