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A kind of production technology of sapindus saponin

Sapindus saponin, a production process technology, applied in the field of medicine and chemical industry, can solve the problems of complex production process of sapindus saponin, unfavorable industrial production, etc., and achieve the effect of easy control of activity, easy regeneration, and cost reduction

Active Publication Date: 2016-08-24
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the defect that the existing sapindus saponin production process is complex and unfavorable for industrialized production, and provides a simple and high-purity sapindus saponin production process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 50g of Sapindus chinensis powder, add 5 times of water-saturated n-butanol, ultrasonically extract 4 times, each time for 15min; , the first 0.25BV was discarded, and the 4BV eluate was collected; the eluate was decolorized twice with 10% activated carbon and ultrasonically decolorized for 20 minutes each time. Sapindus total saponins 6g.

Embodiment 2

[0025] Take 1000g of Sapindus chinensis powder, add 7 times of water-saturated n-butanol, ultrasonically extract 3 times, 20min each time; filter, combine the filtrates, concentrate to dryness, and pass through silica gel partition chromatography. Water was used as the stationary phase, n-butanol was used as the mobile phase, the first 0.25 BV was discarded, and 4 BV of the eluate was collected; the eluate was decolorized by adding 6% activated carbon and ultrasonically decolorized 3 times, each time for 15 min, after filtration, the filtrate was combined and concentrated to Dry it to obtain 12g of Sapindus total saponins with a purity of more than 90% and a white color.

Embodiment 3

[0027] Take 250g of Sapindus chinensis powder, add 6 times of water-saturated n-butanol, ultrasonically extract 4 times, each time for 30min; , the first 0.25BV was discarded, and the 4BV eluate after collection was added; the eluate was decolorized twice with 10% activated carbon and ultrasonically decolorized for 20 minutes each time, and the filtrate was combined after filtration and concentrated to dryness to obtain a pure white color free of over 90% purity. Total saponins 30g.

[0028] Note: The reference substance used in the UV purity test is the sapindoside B isolated from Sapindoside chinensis.

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PUM

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Abstract

The invention belongs to the field of medicine and chemical industry, and discloses a production process of sapindus saponins. The steps include: S1. ultrasonically extracting sapindus powder with n-butanol, combining the filtrates, and washing the filtrates with acid water and alkaline water respectively Concentrate to dryness; S2. Silica gel partition chromatographic column: take the total sapindus sapindus saponins obtained in S1 and purify them by silica gel partition chromatography, using silica gel as a support agent, water as a stationary phase, and n-butanol as a mobile phase, first elute 0.25BV Discard, then elute 4BV, and combine the eluents; S3. Decolorization: Take the eluent from S2, add activated carbon for ultrasonic decolorization, filter and combine the filtrates, concentrate to dryness, and obtain Sapindus saponins with a purity of 90~95% . The method can quickly and easily obtain high-purity sapinberry saponin from sapindus fruit pulp, adopts a conventional process, and has low cost, and provides a new method for industrial production of high-purity sapinberry saponin.

Description

technical field [0001] The invention relates to the field of medicine and chemical industry, in particular to a production process of high-purity sapindus total saponins. Background technique [0002] Sapindus saponin has anti-dermatophyte, candida and anti-helicobacter pylori activities, is a natural sterilant, and has various biological activities such as inhibiting tumor cell proliferation and anticoagulation, and has good application prospects in the field of medicine . Sapindus saponin is also a pure natural surfactant, with a pH value between 5 and 7, which is naturally acidic, rich in foam, delicate in hand, and strong in detergency. It is the best natural cleaning material in the world. Soapberry saponin can be completely degraded, does not harm the human body, does not pollute water sources, and can be used to make natural pollution-free cleaning products. This type of natural plant cleaning products has become quite popular in Japan, South Korea, and developed co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/06
Inventor 蒋林陈媛李敬高玉婕吴亚萍
Owner SUN YAT SEN UNIV
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