Plant tissue culture medium added with sodium hypochlorite for sterilization instead of high temperature and culture method

Abstract

The invention provides a plant tissue culture medium added with sodium hypochlorite for sterilization instead of high temperature and a culture method. Every 1000 ml of the plant tissue culture medium comprises the components of the conventional culture medium and 10 to 40 g of sodium hypochlorite. According to the invention, sodium hypochlorite is added to the plant tissue culture medium for sterilization instead of high temperature, so that adverse effects on the plant tissue culture medium by high temperature sterilization can be avoided, and the production efficiency of the plant tissue culture medium can be increased; since the conventional plant tissue culture medium is added with sodium hypochlorite, high temperature sterilization is not needed to perform to the culture medium and the plants can grow advantageously; since the pH value is 5.8 to 6.0, sodium hypochlorite can serve to sterilize advantageously, the sterilization effect is good, and the plants in tissue culture are prevented from being polluted; the operation is simple, the sterilization can be achieved in a short time, the production efficiency of the culture medium can be increased, the culture cost can be reduced, and an efficient culture method can be provided for carrying out a large-scale plant tissue culture.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and particularly relates to a plant tissue culture medium and a culture method in which sodium hypochlorite is added instead of high-temperature sterilization. The plant tissue culture medium per 1000 ml contains conventional medium components and 10-40 mg of sodium hypochlorite. Background technique [0002] Plant tissue culture technology originated in the 20th century and has a history of more than 100 years. Plant tissue culture technology has become one of the most commonly used biotechnology methods. Plant tissue culture technology is an integration of many technologies, among which sterilization technology is one of the key technologies in the tissue culture process. Sterilization methods include high-temperature sterilization, filter sterilization, ethanol sterilization, etc., wherein high-temperature sterilization includes high-temperature steam sterilization, dry heat sterilization,...

AI Technical Summary

Problems solved by technology

However, high-pressure steam sterilization has the following disadvantages: more expensive equipment; more energy consumption; longer sterilization time; during the high-temperature steam sterilization process, some chemical reactions will occur in the medium, and the components of the medium will be accompanied by Some changes, such as 59% of lysine, arginine and other basic amino acids were destroyed at 121°C for 20 minutes, and a considerable amount of methionine and tryptophan were also destroyed; the sterilized quantity of the medium was limited, Higher production costs etc.

Although this method uses sodium hypochlorite for the sterilization of plant tissue culture medium, the plant tissue culture medium still needs to be subjected to high-temperature sterilization before it can be used for plant tissue culture, and high-temperature sterilization cannot be avoided, especially high-pressure steam sterilization. Shortcomings

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