Use of usnic acid and derivatives of usnic acid for preparation of medicaments for treating skin diseases caused by malassezia

A skin disease, Malassezia technology, applied in skin diseases, skin care preparations, antibacterial drugs, etc., can solve problems such as the efficacy of usnic acid skin diseases and its drugs that have not been reported, and achieve convenient access to materials , the effect of broad application prospects

Active Publication Date: 2012-07-04
晟薇药业(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Taking a comprehensive view of the research at home and abroad, there is no report on the efficacy of usnic acid and its derivatives in the prevention and treatment of skin diseases caused by Malassezia and its medicinal properties.

Method used

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  • Use of usnic acid and derivatives of usnic acid for preparation of medicaments for treating skin diseases caused by malassezia
  • Use of usnic acid and derivatives of usnic acid for preparation of medicaments for treating skin diseases caused by malassezia
  • Use of usnic acid and derivatives of usnic acid for preparation of medicaments for treating skin diseases caused by malassezia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Usnic acid inhibits Malassezia

[0032] Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344);

[0033] Medium: Malassezia solid medium: glucose 40 g / L, agar 14 g / L, peptone 10 g / L, dissolved in distilled water, adjusted to pH 6.8-7.0, autoclaved at 115°C for 20 min; Malassezia Bacteria liquid medium: glucose 40 g / L, dissolved in distilled water, adjusted to pH 6.8-7.0, autoclaved at 115°C for 20 min.

[0034] Experimental method: After the test bacteria were subcultured on the Malassezia solid medium, to ensure the purification and viability of the colony, pick a single clone into 50 ml Malassezia liquid medium, culture it at 30°C for 24 h, and use Turbidimeter (Biological Mérieux Company) was adjusted to the concentration of McFarland No. 4 turbidimetric tube. Dilute the bacterial solution with medium to make the final bacterial concentration 10 5 CFU / mL, then draw 100 ul and apply it on the solid medium, and use the tube and plate meth...

Embodiment 2

[0038] Example 2 Determination of Usnea Sodium Inhibiting Malassezia MIC Value

[0039] Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344);

[0040] Medium: Malassezia liquid medium: glucose 40 g / L, dissolved in distilled water, adjusted to pH 6.8-7.0, autoclaved at 115°C for 20 min.

[0041] Experimental method: After the test bacteria were subcultured on the Malassezia solid medium, to ensure the purification and viability of the colony, pick a single clone into 50 ml Malassezia liquid medium, culture it at 30°C for 24 h, and use Turbidimeter (Biological Mérieux Company) was adjusted to the concentration of McFarland No. 4 turbidimetric tube. Sodium usnicate was directly diluted with Malassezia liquid culture medium to test concentrations, which were 12.5, 6.25, 3.125, 1.6, 0.8, 0.4, 0.2, and 0.1 μg / ml. Add 100 μL of bacterial solution and 100 μL of medicinal solution to the microwells of the 96-well plate, and set up a negative control without adding ...

Embodiment 3

[0047] Example 3 Preparation of hair conditioner

[0048] Prepared according to the ratio shown in Table 3,

[0049] table 3

[0050]

[0051] Preparation process: raise the temperature of phase A to 80~85°C, stir evenly; raise the temperature of phase B to 80~85°C, stir evenly; add phase A into phase B, homogenize for 3 minutes; cool down and stir; cool down to below 40°C, Add phase C and stir well to make conditioner.

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Abstract

The invention belongs to the field of pharmaceuticals, and relates to the new use of an usnic acid and derivatives of the usnic acid, specifically to the use of the usnic acid and the derivatives of the usnic acid for the preparation of medicaments for treating skin diseases caused by malassezia, in particular to the use of the usnic acid and the derivatives of the usnic acid for the preparation of medicaments for treating overmuch scurf, seborrheic dermatitis, atopic dermatitis and psoriasis caused by the malassezia. Inhibition test results show that an average diameter value of an inhibition zone is 20mm and the usnic acid can remarkably inhibit the growth of the malassezia; and a measurement result of a minimal inhibitory concentration (MIC) value of a sodium usnic acid for the inhibition of the malassezia shows that the growth of the malassezia is inhibited along with the rise of a medicinal concentration gradient of the usnic acid and the derivatives of the usnic acid. The usnic acid and the derivatives of the usnic acid disclosed by the invention can be used for preparing the medicaments for treating the skin diseases caused by the malassezia, and can also be used for preparing household chemicals and external preparations for preventing and treating the skin diseases.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to the new application of usnic acid and its derivatives. It specifically relates to the use of usnic acid and its derivatives in the preparation of medicines for the treatment of skin diseases caused by Malassezia, in particular to the use of usnic acid and its derivatives in the preparation and treatment of excessive dandruff and seborrheia caused by Malassezia Use in medicines for atopic dermatitis, atopic dermatitis and psoriasis. Background technique [0002] Usnenic acid is the dried lichen extract of Usnea diffracta Vain. and Usnea longissima Ach. of Usneaceae (Usneaceae) plants; Usnea acid is also called lichenic acid, and its English name is: Usninic acid; Molecular formula: C 18 h 16 o 7 ; Chemical name: 1,3(2H,9bH)-Dmenzofurandione, 2,6-diaacetyl-7, 9-dihydroxy-8, 9b-dimethyl; molecular weight: 344.1, physical and chemical properties: usnic acid has optical activity , found in li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61K9/12A61P31/04A61P17/00A61P17/08A61P17/06A61K8/49A61Q5/02A61Q5/12A61Q19/00A61Q19/10
Inventor 孙常磊翟春涛朱丽平李成亮
Owner 晟薇药业(上海)有限公司
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