Microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon

A microbial agent and polycyclic aromatic hydrocarbon technology, which is applied in the direction of microorganisms, microbial-based methods, biological water/sewage treatment, etc., can solve the problems that the remediation effect of polycyclic aromatic hydrocarbon-contaminated soil has not been reported, and achieve good application prospects.

Inactive Publication Date: 2012-07-04
CHINA AGRI UNIV
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Problems solved by technology

Mycobacterium is an important group of microorganisms with a strong ability to degrade PAHs with four or more rings. The screening of mycobacterial strains d

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  • Microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon
  • Microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon

Examples

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[0039] Experimental Example 1 Experiment on degradation of polycyclic aromatic hydrocarbon-pyrene (Pyr) by the microbial agent of the present invention

[0040] According to the ratio of viable bacteria of Mycobacterium flavum P6 and slightly acidophilic oligotrophomonas P56 of 1-2:1, take P6 and P56 bacteria solution totaling 0.5ml bacterial solution into 20mL inorganic salt liquid medium (Na 2 HPO 4 .12H 2 O 8.8g, KH 2 PO 4 3.0g, NH 4 NO 3 1.0g, MgSO 4 .7H 2 O0.246g, FeCl 3 0.05g, CaCl 2 0.02g, trace element solution, 2.5ml, 1000ml distilled water. The composition of the trace element solution is: MnSO 4 .2H 2 O 0.06g, H 3 BO 3 0.03g, CoCl 2 .6H 2 O 0.04g, CuCl 2 .2H 2 O 0.01g, NiCl 2 .6H 2 O 0.02g, Na 2 MoO 4 .2H 2 O 0.03g, ZnCl 2 0.05g, pH7, 1000ml distilled water) serum bottle, pre-added 5g / L pyrene acetone solution 0.1ml in the serum bottle, and volatilize the acetone, the final concentration of fluoranthene in the medium is 50mg / L, avoid 30℃ Light shaker culture, samples we...

Example Embodiment

[0042] Experimental Example 2 Experiment on degradation of polycyclic aromatic hydrocarbon-pyrene (Pyr) and fluoranthene (Flu) mixture by the microbial agent of the present invention

[0043] Change the concentration of polycyclic aromatic hydrocarbons in the serum bottle, add 0.1ml of 5g / L fluoranthene in acetone; at the same time add 0.1ml of 5g / L pyrene in acetone, and evaporate the acetone so that the medium contains pyrene at the same time And fluoranthene, their final concentration was 100mg / L, respectively, after inserting the degrading strains, cultured in a shaker at 30°C and protected from light, samples were taken on the 7th and 14th days to determine the remaining polycyclic aromatic hydrocarbons in the culture solution. The negative control CK is an inorganic salt medium with only pyrene or fluoranthene added without bacteria.

[0044] The result is figure 2 As shown, the addition of fluoranthene did not affect the degradation of pyrene by the bacterial strain, and t...

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Abstract

The invention provides a microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon. The microorganism bacterium agent for degrading the polycyclic aromatic hydrocarbon consists of light yellow mycobacteria P6, and stenotrophomonas acidaminiphila P56. The bacterium agent has the capacity of degrading multiple kinds of polycyclic aromatic hydrocarbon of soil and sewage, an obvious effect of repairing soil polluted by the polycyclic aromatic hydrocarbon, and good application prospect; and the capacity of degrading polycyclic aromatic hydrocarbon (pyrene and fluoranthene), which are dissolved in a solution, by the microorganism bacterium agent is over 90 percent.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, in particular to a polycyclic aromatic hydrocarbon-degrading microbial bacterial agent containing mycobacterium light flavinum and microacidophilic stenotrophomonas. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to hydrocarbon compounds composed of two or more benzene rings linked together, many of which have carcinogenic, teratogenic, and mutagenic effects, and their toxicity and carcinogenicity increase with the number of benzene rings And increase. PAHs in the environment mainly come from human activities, such as petroleum refining, transportation, and gas production. Due to the extensive use of petroleum and petroleum products, polycyclic aromatic hydrocarbons in the environment tend to increase and are widely distributed. The existence of various PAHs can be detected in the atmosphere, water, soil, sediment and food. [0003] The polycyclic aromatic ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/32C02F101/32
Inventor 宫小燕陆雅海
Owner CHINA AGRI UNIV
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