Manufacturing method of slide for observing filamentous fungus microscopic morphology

Abstract

The invention relates to a manufacturing method of a slide for observing filamentous fungus microscopic morphology. The method comprises the following steps of: firstly digging a groove on a water agar culture flat plate and removing a culture medium in the groove; then placing a water agar culture block with a size of less than that of the groove in the groove, picking up a fungus culture to be identified and inoculating on the upward side of the water agar culture block in the groove and then covering the water agar culture block with a sterile cover glass; and closing a culture dish cover, sealing and culturing at room temperature, taking the cover glass out after the culture is finished and manufacturing an observation slide for fungus. The method provided by the invention is easy in operation for manufacturing a thallus observation slide of filamentous fungus; observation slides for thalli at different development stages can be obtained by using a continuous slide taking method according to growth periods of different thalli; and the accuracy for identification work of the filamentous fungus species is guaranteed.

Description

technical field [0001] The invention belongs to the field of mycological research, and in particular relates to a method for making slices for observing the microscopic morphology of filamentous fungi. Background technique [0002] For a long time, the biological identification of filamentous fungi is mainly based on the morphological characteristics of the fungi. Therefore, it is very important to obtain a good microscopic observation slide of the fungi to accurately identify the species of filamentous fungi. At present, the steps of the traditional method of making filamentous fungal observation slides are as follows: first, use a pick needle to directly pick the fungal culture on the medium and place it on a glass slide; then carefully remove the picked mycelium with a pick needle Spread out and cover with a coverslip. However, this method has the following obvious disadvantages: (1) Because the traditional method of producing slides is prone to overlap of bacteria, it i...

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Problems solved by technology

However, this method has the following obvious disadvantages: (1) Because the traditional method of producing slides is prone to overlap of bacteria, it is difficult to obtain slides with good observation effect. Even skilled operators need a large number of slides to obtain slides. Observation slides that meet the observation requirements; (2) During the picking process, the natural growth state of fungal spores on the spore carrier is easily destroyed by human beings. Therefore, the observation slides obtained by the traditional picking method are often difficult to reflect The sporulation mode and type of fungi, and this feature is the most critical identification factor in the classification of filamentous fungi; (3) In the observation slides obtained by picking slides, the bacteria are often in the late stage of development. It is impossible to obtain early-stage fungi, which is very unfavorable for studying the morphological characteristics of fungi at different developmental stages