Method for detecting restaurant and kitchen waste oil mixed in edible vegetable oil
A technology of edible vegetable oil and detection method, applied in the detection field of kitchen waste oil, can solve the problems of surfactant residue, misjudgment, poor thermal stability, etc.
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Embodiment 1
[0092] Comparison of polyglyceride content in different samples.
[0093] Detection method of polyglyceride content:
[0094] One), the extraction of oil polar components
[0095] Method 1) Ordinary column chromatography: first accurately weigh 2.5 g (M) of the oil sample, dissolve it completely with 20 mL of petroleum ether with a boiling range of 30 to 60 °C, and then dilute to volume with petroleum ether with a boiling range of 30 to 60 °C. 50mL, take 20mL of the sample solution and put it on a silica gel chromatography column (a glass column with an inner diameter of 21mm and a length of 450mm, filled with 25 grams of 100-200 mesh silica gel with a water content of 5%), and wash with 150mL of washing solution (30-60℃ Petroleum ether in the boiling range: diethyl ether = 87:13) wash the silica gel chromatography column at a flow rate of 2 to 2.5 mL / min, discard the washing solution; then use 150 mL of diethyl ether to elute the silica gel layer at a flow rate of 2 to 2.5 m...
Embodiment 2
[0136] Comparison of oxidized glyceride content in different samples.
[0137] Detection method of oxidized glyceride content:
[0138] One), the extraction of oil polar components
[0139] Method 1) Ordinary column chromatography: first accurately weigh 2.5 g (M) of the oil sample, dissolve it completely with 20 mL of petroleum ether with a boiling range of 30 to 60 °C, and then dilute to volume with petroleum ether with a boiling range of 30 to 60 °C. 50mL, take 20mL of the sample solution and put it on a silica gel chromatography column (a glass column with an inner diameter of 21mm and a length of 450mm, filled with 25 grams of 100-200 mesh silica gel with a water content of 5%), and wash with 150mL of washing solution (30-60℃ Petroleum ether in the boiling range: diethyl ether = 87:13) wash the silica gel chromatography column at a flow rate of 2 to 2.5 mL / min, discard the washing solution; then use 150 mL of diethyl ether to elute the silica gel layer at a flow rate of ...
Embodiment 3
[0156] Comparison of low carbon number (≤C14) fatty acid content in different samples.
[0157] Detection method of low carbon number (≤C14) fatty acid content:
[0158] One), the extraction of oil polar components
[0159] Method 1) Ordinary column chromatography: first accurately weigh 2.5 g (M) of the oil sample, dissolve it completely with 20 mL of petroleum ether with a boiling range of 30 to 60 °C, and then dilute to volume with petroleum ether with a boiling range of 30 to 60 °C. 50mL, take 20mL of the sample solution and put it on a silica gel chromatography column (a glass column with an inner diameter of 21mm and a length of 450mm, filled with 25 grams of 100-200 mesh silica gel with a water content of 5%), and wash with 150mL of washing solution (30-60℃ Petroleum ether in the boiling range: diethyl ether = 87:13) wash the silica gel chromatography column at a flow rate of 2 to 2.5 mL / min, discard the washing solution; then use 150 mL of diethyl ether to elute the s...
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