Method for preparing anti-inflammatory and anti-tumor active ingredient group from liquorice dregs and application thereof

A technology of active ingredients and licorice dregs, which is applied in the preparation of anti-inflammatory and anti-tumor active ingredient groups from licorice dregs and its application field, can solve the problems of shortage of licorice resources and increasing demand for licorice, and achieve composition Clear, clear principal component content, the effect of inhibiting the growth of tumor cells

Active Publication Date: 2013-10-30
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the demand for licorice has been increasing because of its remarkable pharmacological effects and wide range of uses. Therefore, the shortage of licorice resources has become increasingly prominent and has gradually become a major problem.

Method used

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  • Method for preparing anti-inflammatory and anti-tumor active ingredient group from liquorice dregs and application thereof
  • Method for preparing anti-inflammatory and anti-tumor active ingredient group from liquorice dregs and application thereof
  • Method for preparing anti-inflammatory and anti-tumor active ingredient group from liquorice dregs and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) 10kg of dried licorice residue (obtained after water extraction of glycyrrhizic acid) was refluxed with 95% ethanol for three times, 2 hours, 2 hours, 1.5 hours, and the extract was recovered under reduced pressure to obtain crude ethanol extract;

[0028] (2) The ethanol extract was suspended in 5L of distilled water, and extracted four times with equal volumes of petroleum ether and ethyl acetate;

[0029] (3) The ethyl acetate extract obtained in step (2) was separated by silica gel column chromatography, and eluted with petroleum ether: acetone 10:1, 2:1, 1:1,

[0030] (4) The petroleum ether: acetone 2:1 fraction obtained in the above step (3) was separated by polyamide column chromatography, and ethanol / water 2:8, 6:4, 9:1 gradient elution, ethanol / water 6: 4 The eluate is the active ingredient group.

[0031] (5) Through HPLC analysis and standard comparison method, the composition of the obtained product was clarified (the structures of S1-S13 are shown in ...

Embodiment 2

[0042] (1) 5 kg of dried licorice residue (obtained after extracting glycyrrhizic acid by alkaline water method) was reflux extracted with methanol, and the extract was recovered under reduced pressure to obtain crude methanol extract;

[0043] (2) The methanol extract was suspended in 1L of distilled water, separated and purified by HPD100 macroporous adsorption resin, and then eluted with ethanol / water mixed solvent ratios of 3:7, 6:4, and 9:1, and the ratio of ethanol / water mixed The crude product was obtained as a 6:4 partial eluate

[0044] (3) The crude product obtained in step (2) is separated by polyamide column chromatography, and eluted successively with ethanol / water mixed solvent ratios of 2:8, 4:6, 6:4, and 8:2, and the mixing ratio of ethanol / water is 4:6~6:4 part of the eluate obtained the target active ingredient group.

[0045] (4) The composition of the obtained product was clarified through HPLC analysis and standard comparison method, and the experimental me...

Embodiment 3

[0048] Quinone reductase-inducing activity test of active ingredient group

[0049] 1. Experimental materials:

[0050] (1) Main reagents and main equipment: calf serum (purchased from Hyclone); fetal bovine serum (TBD);

[0051] Tetramethylazolium salt (MTT) American Sigma (St. Louis, MO); Glucose-6-phosphate dehydrogenase (Shanghai Sangong); 96-well cell culture plate (Costar); cell line: mouse liver cancer cell Strain Hepa 1c1c7

[0052] 2. Experimental method:

[0053] (1) Cell culture:

[0054] Seed 10 per hole 4 Cells were grown for 24 hours in a culture medium containing 10% (v / v) fetal bovine serum, 0.01% penicillin G, 0.15% sodium bicarbonate, 0.01% streptomycin sulfate, the environmental conditions were 37 ℃, containing 5% CO 2 of humid air.

[0055] (2) Preparation of drugs

[0056] The compound to be tested was dissolved in DMSO to make a mother solution with a concentration of 50mmol / L, and stored at -20°C.

[0057] (3) Crystal violet method t...

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Abstract

The invention provides a method for preparing an anti-inflammatory and anti-tumor active ingredient group from liquorice dregs and an application of the anti-inflammatory and anti-tumor active ingredient group. The anti-inflammatory and anti-tumor active ingredient group prepared from the liquorice dregs comprises 13 flavone compounds; and the method for preparing the anti-inflammatory and anti-tumor active ingredient group from the liquorice dregs comprises the following steps: drying liquorice dregs; crushing the liquorice dregs; by using a solvent extracting method, extracting the liquorice dregs by ethanol or methanol; decompressing and recycling an extraction liquor so as to obtain a crude extract; suspending the crude extract by a distilled water so as to obtain an extract suspension; extracting the extract suspension by petroleum ether or cyclohexane and ethyl acetate in sequence; separating an ethyl acetate extract by silica gel column chromatography; eluting the separated ethyl acetate extract by petroleum ether / ethyl acetate or petroleum ether / acetone mixed solvent in a gradient way; separating an obtained fraction by polyamide column chromatography; and eluting the separated fraction by methanol / water or ethanol / water as mobile phase so as to obtain the active ingredient group. The anti-inflammatory and anti-tumor active ingredient group prepared by using the method provided by the invention, has the advantages of explicit composition, effects of suppressing the growth, proliferation and migration of tumor cell, quinone reductase inducing effect, tumor angiogenesis suppressing effect and anti-inflammatory effect.

Description

technical field [0001] The invention relates to a method for preparing anti-inflammatory and anti-tumor active ingredients from licorice dregs in the technical field of medicine and its application. Background technique [0002] Licorice (Licorice) is also known as sweet grass, honey grass, beautiful grass, etc., and its medicinal part belongs to the genus Licorice (Leguminosae). Glycyrrhiza ) roots and rhizomes of plants. Glycyrrhiza licorice is widely distributed in my country, with large reserves and remarkable pharmacological effects. It is one of the most commonly used medicinal plants in my country and has been the focus of research by domestic and foreign researchers for many years. At the same time, the demand for licorice has been increasing only because of its remarkable pharmacological effects and wide range of uses. Therefore, the shortage of licorice resources has become increasingly prominent and has gradually become a major problem. At present, pharmaceutica...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61K31/12A61P29/00A61P35/00A61P35/04A61P43/00
Inventor 李宁马忠俊
Owner SHENYANG PHARMA UNIVERSITY
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