Lignin degrading enzyme as well as preparation method and application thereof

A technology of lignin degrading enzyme and lignin, which is applied in the field of lignin degrading enzyme and its preparation, and the application of lignin degrading enzyme, which can solve the problems of few reports

Active Publication Date: 2014-12-03
GUANGDONG GOLDEN LEAF TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many reports on this aspect, and there are few reports on the industrial production of lignin-degrading enzyme preparations and their application in the treatment of cut tobacco stems.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1: Preparation of Lignin Degrading Enzyme Using Shredded Tobacco Stems

[0094] The fermentation strain is a white rot fungus CICC 40299 strain, and its bacterial agent is prepared by culturing according to the method described in the manual. The shredded tobacco stems obtained by rinsing with water, cutting stems, flash steaming, and puffing and drying were pretreated at a temperature of 90° C. for 2 hours, and the obtained solid-liquid mixture was used as a part of fermentation medium. Make the amount of shredded tobacco stems reach 2g / L, and at the same time make glucose reach 10g / L, ammonium tartrate 0.05g / L, urea 0.05g / L, veratrol 5mmol / L, trace element mixture 1mL / L, pH natural, The sample volume of the shaker flask is 40% of the volume of the 250mL Erlenmeyer flask, the inoculation volume of the white rot fungus CICC 40299 is 10% of the medium volume, the culture temperature is 30°C, the rotation speed is 150r / min, and the culture time is 10 days. Centri...

Embodiment 2

[0096] Example 2: Preparation of Lignin Degrading Enzyme Using Shredded Tobacco Stems

[0097] The fermentation strain is a white rot fungus CICC 40299 strain, and its bacterial agent is prepared by culturing according to the method described in the manual. The shredded tobacco stems obtained by rinsing with water, cutting stems, flash steaming, and puffing and drying were pretreated at a temperature of 90° C. for 3 hours, and the obtained solid-liquid mixture was used as a part of fermentation medium. Make the amount of shredded tobacco stems reach 10g / L, and at the same time make glucose reach 20g / L, ammonium tartrate 0.30g / L, urea 0.30g / L, veratrol 5mmol / L, trace element mixture 1mL / L, pH natural, The sample volume of the shaker flask is 40% of the volume of the 250mL Erlenmeyer flask, the inoculation volume of the white rot fungus CICC 40299 is 10% of the medium volume, the culture temperature is 30°C, the rotation speed is 150r / min, and the culture time is 10 days. Centr...

Embodiment 3

[0098] Example 3: Preparation of Lignin Degrading Enzyme Using Shredded Tobacco Stems

[0099] The fermentation strain is a white rot fungus CICC 40299 strain, and its bacterial agent is prepared by culturing according to the method described in the manual. The shredded tobacco stems obtained by rinsing with water, cutting stems, flash steaming, and puffing and drying were pretreated by boiling at a temperature of 90°C for 2.5 hours, and the obtained solid-liquid mixture was used as a part of fermentation medium. Make the amount of shredded tobacco stems reach 5g / L, and at the same time make glucose reach 20g / L, ammonium tartrate 0.25g / L, urea 0.15g / L, veratrol 5mmol / L, trace element mixed solution 1mL / L, pH natural, The sample volume of the shaker flask is 40% of the volume of the 250mL Erlenmeyer flask, the inoculation volume of the white rot fungus CICC 40299 is 10% of the medium volume, the culture temperature is 30°C, the rotation speed is 150r / min, and the culture time i...

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Abstract

The invention relates to a lignin degrading enzyme as well as a preparation method and application thereof. The preparation method of the lignin degrading enzyme comprises the steps of tobacco stem shred boiling in water, liquid fermentation, centrifugal separation, ultrafiltration concentration, dialysis treatment, column chromatography purification and the like. Compared with the exiting other methods, the production method of the tobacco stem shred lignin degrading enzyme has the beneficial effects that degraded tobacco stem shred lignin having higher enzyme activity and high pertinence is produced; and the method can be directly applied to pretreatment of stem shreds.

Description

【Technical field】 [0001] The invention relates to the technical field of tobacco processing. More specifically, the present invention relates to a lignin-degrading enzyme and a preparation method thereof, and also relates to the use of the lignin-degrading enzyme. 【Background technique】 [0002] The main components of shredded tobacco stems are lignin, cellulose, and pectin, and lignin is the main reason for the miscellaneous gas, woody gas and throat burning sensation of shredded tobacco stems when burning. [0003] Lignin is an aromatic polymer compound that strengthens cell walls and binds fibers by forming an interwoven network, and has anti-compression effects. [0004] Lignin is divided into syringyl lignin (S-lignin), guaiacyl lignin (G-lignin) and p-hydroxy-phenyl lignin (H-hydroxy-phenyl lignin, H- lignin). [0005] Lignin in tobacco cell wall is a three-dimensional network substance with complex structure composed of phenylpropane derivative monomers (such as co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00C12N9/08C12N9/02A24B15/20C12R1/645
Inventor 郭春梅张建栋施栩翎
Owner GUANGDONG GOLDEN LEAF TECH DEV
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