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Simple tissue culture method for broussonetia papyrifera

A simple and tissue-cultivating technology, applied in the field of plant tissue culture, can solve the problems of high manual operation cost, high energy consumption of aseptic disinfection, etc., and achieve the effect of reducing tissue culture cost, reducing workload and energy consumption, and strong practicability

Inactive Publication Date: 2013-01-02
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional mulberry tissue culture methods require the inoculation process and the culture process to be carried out in sterile medium, which consumes a lot of energy for aseptic disinfection and high manual operation costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: A simple tissue culture method of Broussonetia papyrifera

[0036] A simple tissue culture method of paper mulberry, including the following steps:

[0037] 1. Preparation of bacteriostatic agent: Preparation method of bacteriostatic agent No. 1: Add 20 ml of distilled water to bottle A of Eperilon fungicide and mix it with bottle B of medicament, and dilute the volume to 100 ml with distilled water for bacteriostasis Preparation method of No. 1; Bacteriostat No. 2: Add 20 ml of distilled water to bottle A of Eperilon fungicide and mix it with bottle B, then add 15 ml of sodium hypochlorite solution, and dilute to 100 ml with distilled water. Bacteriostatic agent No. 2;

[0038] 2. Disinfection of the culture container: soak the culture bottle and the cap in an aqueous solution with a volume ratio of bacteriostatic agent No. 1 to water of 0.4% (V / V) for 10 hours, and store for later use;

[0039] 3. Medium preparation: the induction medium is MS+0.8 mg / L 6-BA+0.1 ...

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PUM

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Abstract

The invention relates to a simple tissue culture method for broussonetia papyrifera. The simple tissue culture method for the broussonetia papyrifera comprises the following steps: preparing a bacteriostatic agent, sterilizing a culture vessel, preparing a culture medium, performing induction culture, performing proliferation culture, performing rooting culture and transplanting a bottled plantlet. By the simple tissue culture method for the broussonetia papyrifera, the culture vessel and the culture medium are not required to be sterilized at high temperature and high pressure, so that the workload and the energy consumption are reduced, the tissue culture link of the broussonetia papyrifera is simplified, and tissue culture cost of the broussonetia papyrifera is lowered; the simple tissue culture method for the broussonetia papyrifera is easy to operate, high in practicality and good in popularization, and the culture medium is prepared according to different formulas and the bacteriostatic agent is added; and compared with the conventional tissue culture method for the broussonetia papyrifera, by the simple tissue culture method for the broussonetia papyrifera, the cost can be reduced by more than 10%.

Description

Technical field [0001] The invention relates to a plant tissue culture method, in particular to a simple tissue culture method of Broussonetia papyrifera, belonging to the field of biotechnology. Background technique [0002] Paper mulberry ( Broussonetia papyriferaL ) Belongs to the Moraceae family, is a perennial broad-leaved tree with the characteristics of fast growth, strong adaptability, wide distribution, high heat, and short rotation period. Broussonetia papyrifera is a special economic forest tree species with low investment, short cycle, quick effect and long benefit. It is mainly distributed in the Yellow River, Yangtze River and Pearl River basins in my country. The phloem of Broussonetia papyrifera has high fiber content, long and thin fibers, and good quality. It is a high-quality raw material for the production of rice paper and coinage paper. The leaves are rich in protein, amino acids, vitamins, carbohydrates and trace elements, and are purely natural for process...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 阙友雄林庆良许莉萍高世武
Owner FUJIAN AGRI & FORESTRY UNIV