Method for making ceramide formation accelerator
A technology of ceramides and manufacturing methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of improving moisturizing ability
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Embodiment 1
[0046]
[0047] First, the culture medium is prepared according to the following method: Soak soybeans in water for 12 hours to allow the soybeans to fully absorb water, then grind and crush the absorbed soybeans, filter them to remove liquid components, and drain the remaining residues to obtain bean dregs. The okara was mixed with glucose and water, and the concentrations of the okara and glucose in the mixture were respectively 10w / v% and 1w / v%, and the mixture was used as a culture medium.
[0048] Then, add 100ml of the above-mentioned medium into the Erlenmeyer flask, inoculate a certain amount of Bacillus subtilis (preserved in CICIM, Industrial Microbiology Resource and Information Center of Jiangnan University, No. CICIM B1677), and cultivate at 30°C for 3 days .
[0049] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times of absolute ethanol, and the mixture diluted with absolute ethanol was ultra...
Embodiment 2
[0067] In the preparation process of the ceramide / glucosylceramide production accelerator of Example 1, as a microorganism of the genus Bacillus (Bacillus), Bacillus subtilis (Bacillus subtilis) (preserved in Jiangnan University Industrial Microbiology Resources and Information Center CICIM, No. CICIM B1923), except that it was cultured in the same manner as in Example 1.
[0068] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times of absolute ethanol, and the mixture diluted with absolute ethanol was ultrasonically treated for 10 minutes, centrifuged (3000rpm) for 30 minutes, and the supernatant (about 70ml) was collected. The filtrate was obtained by filtration, and the filtrate was dried in a vacuum dryer to constant weight. The dried product was used as samples C and D of the ceramide / glucosylceramide production accelerator of the present invention, and samples C and D were dissolved in 50% ethanol respect...
Embodiment 3
[0072] In the preparation process of the ceramide / glucosylceramide production accelerator of Example 1, as a microorganism of the genus Bacillus (Bacillus), Bacillus acidicola (Bacillus acidicola) (preserved in Jiangnan University Industrial Microbiology Resources and Information Center CICIM , No. CICIM B1297), except that it was cultured in the same manner as in Example 1.
[0073] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times absolute ethanol, and 1ml of the mixture was taken as sample E of the ceramide / glucosylceramide production accelerator of the present invention, and stored in a 4°C refrigerator for use as an additive for cell culture.
[0074] The above-mentioned mixed solution diluted with absolute ethanol was ultrasonically treated for 10 minutes, centrifuged (3000rpm) for 30 minutes, and the supernatant (about 70ml) was collected. The supernatant was filtered with filter paper to obtain the f...
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