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Method for improving yield of nata de coco

A technology of coconut fiber fruit and yield, applied in the application, food preparation, food science and other directions, can solve the problems of low coconut fiber fruit yield, insufficient supply of raw materials, poor recycling effect of fermentation liquid, etc., and achieves important economic benefits and social benefits. Benefit, relieve the excessive reproduction of microorganisms, and facilitate the operation

Active Publication Date: 2012-07-25
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of method that improves coconut fine fruit output for the deficiencies in the prior art, by adding Vc, UDP-Glucose and Na in fermented liquid 2 SO 3 It can improve the antioxidant activity in the fermentation broth, reduce the oxidative stress of the bacterial cells, effectively improve the cellulose synthesis ability of the bacterial cells, and effectively solve the shortage of raw materials in production practice, the low yield of coconut fiber fruit and the recycling of fermentation broth Ineffective and many other problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Get coconut water 5kg (pH 3.5, sugar content 2°Brix) of natural fermentation 5 days and mix with 5kg tap water, add sucrose 340g, (NH 4 ) 2 SO 4 30g, KH 2 PO 4 20g, MgSO 4 5g, mixed well and adjusted to pH 3.8, sterilized at 121°C for 20min; after cooling, add V C 1.40g, UDP-Glucose 0.49g, Na 2 SO 3 2.08g, after mixing, add 500g of Gluconacetobacter xylinum mother liquor; after shallow pan fermentation for 6 days, the yield of coconut fiber fruit is 513.79g (dry weight). Control: No addition of V under other conditions (composition, ratio, inoculum size, fermentation conditions, etc.) C , UDP-Glucose and Na 2 SO 3 , obtain coconut fine fruit output to be 314.24g (dry weight), and the present invention compares with contrast, and output increases 199.55g, increases output by 63.5%.

Embodiment 2

[0029] Get coconut water 3kg (pH 3.6, sugar content 3°Brix) of natural fermentation 7 days, mix with 1.5kg fermentation waste liquid and 5kg tap water, then add sucrose 310g, (NH 4 ) 2 SO 4 20g, KH 2 PO 4 10g, MgSO 4 4g, mix well and adjust to pH 4.0, sterilize at 121°C for 20min; add V C 4.00g, UDP-Glucose 0.80g, Na 2 SO 3 3.20g, after mixing, insert 500g of Gluconacetobacter xylinum mother liquor, and after shallow plate fermentation for 5 days, the coconut fiber fruit output is 487.15g (dry weight). Control: without adding VC, UDP-Glucose and Na 2 SO 3 , obtain coconut fine fruit output to be 243.94g (dry weight), and the present invention compares with contrast, and output increases 243.21g, increases output by 99.7%.

Embodiment 3

[0031] Get naturally fermented coconut water 6kg (pH 3.2, sugar content 3°Brix), mix with 1kg fermentation waste liquid and 3kg tap water, then add sucrose 380g, (NH 4 ) 2 SO 4 40g, KH 2 PO 4 30g, MgSO 4 6g, mixed and adjusted to pH 3.6, sterilized at 121°C for 20min; after cooling, add V C 1.00g, UDP-Glucose 0.10g, Na 2 SO 3 0.80g, after mixing, insert 500g of Gluconacetobacter xylinum mother liquor, and after shallow plate fermentation for 4 days, the coconut fiber fruit output is 527.18g (dry weight). Control: No addition of V under other conditions (composition, ratio, inoculum size, fermentation conditions, etc.) C , UDP-Glucose and Na 2 SO 3 , obtain coconut fine fruit output and be 295.67g (dry weight), the present invention compares with contrast, and output increases 231.51g, increases output by 78.3%.

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PUM

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Abstract

The invention relates to a method for improving the yield of nata de coco. The method comprises the following steps of: mixing naturally fermented coconut water and water, adding sucrose to regulate the Brix sugar degree, regulating the pH value to 3.6-4.0 to form a fermentation solution, and adding (NH4)2SO4, KH2PO4, MgSO4, Vc, UDP-Glucose and Na2SO3 into the fermentation solution; and inoculating 5 percent of gluconacetobacter xylinus culture mother solution to the fermentation solution, standing, culturing for 4 to 6 days, and thus harvesting the nata de coco. The method is simple in process and convenient to operate; by adding the Vc, the UDP-Glucose and the Na2SO3 into the fermentation solution, the antioxidant activity in the fermentation solution can be improved, the oxidative stress of bacterial cells is reduced, the cellulose synthesizing capability of the bacterial cells is effectively improved, and many problems of insufficient raw material supply, low yield of the nata de coco, poor recycling effect of the fermentation solution and the like in production practice are effectively solved; and the method has important economic benefits and social benefits for sustainable development of the nata de coco industry.

Description

technical field [0001] The present invention relates to a kind of coconut fine fruit production method, specifically relate to a kind of by adding appropriate amount of V C Compositions such as the method for improving coconut fine fruit output. Background technique [0002] Coconut fiber fruit (Nata de coco) is an edible fiber gel thick film grown on the surface of the culture medium through bacterial fermentation with coconut water or coconut juice as raw material. Its main component is a unique gelatinous property of bacterial cellulose. Compared with plant cellulose, bacterial cellulose has the characteristics of high water holding capacity, high tensile strength, and high biological adaptability, and has been widely used as a new type of biomaterial in food, medicine, papermaking, acoustic materials and other fields. Because coconut fibrous fruit is rich in cellulose, has high water holding capacity, good elasticity, smooth taste and good chewiness, it has health func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/068A23L21/15
Inventor 陈卫军黄翊鹏王永夏秋瑜李枚秋赵松林
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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