Molecular marker of wheat powdery mildew-resistant gene Pm45 and application of molecular marker

A powdery mildew resistance gene and molecular marker technology, applied in the field of molecular genetics, can solve problems such as loss of resistance of disease resistance genes, and achieve the effects of stable amplification, convenient detection and accelerated utilization

Inactive Publication Date: 2012-07-25
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the race-specific quality resistance of powdery mildew and the co-evolution of the avirulent gene and the resistance gene of the pathogen, the large-scale promotion of

Method used

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  • Molecular marker of wheat powdery mildew-resistant gene Pm45 and application of molecular marker
  • Molecular marker of wheat powdery mildew-resistant gene Pm45 and application of molecular marker
  • Molecular marker of wheat powdery mildew-resistant gene Pm45 and application of molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) D57 and Yangmai 158F 2 Generation generation and phenotyping:

[0066] (1) Common wheat germplasm D57 Hybrid F obtained by crossing with Yangmai 158(♀) 1 , F 1 F 2 group.

[0067] (2)F 2 The single plant of the generation group is planted in the plug tray, placed in the greenhouse, 14 hours at 22 degrees during the day, and 10 hours at 18 degrees at night. Powdery mildew was inoculated at the stage of one leaf and one heart, and the resistance investigation was carried out 7 days after inoculation. The identification results are shown in Table 1.

[0068] (3)F 2 After the investigation of the generation single plant, the seedlings were transplanted to the field, and the seeds obtained by selfing were F 3 family lineage. each F 3 18 grains were selected in the family for offspring testing. Table 1 for identification results.

[0069] Table 1 Yangmai 158×D57F after inoculation with Bgt18 and Bgt19 2 and F 2∶3 resistance segregation

[0070]

[0071...

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Abstract

The invention belongs to the field of molecular genetics and relates to a molecular marker of a wheat powdery mildew-resistant gene Pm45 and application of molecular marker. The molecular marker is selected from any one of MAG6139, MAG6140 and BARC123, and the genetic distance from the MAG6139, MAG6140 and BARC123 to the Pm45 is 0.7cm, 2.7cm and 5.2cm respectively. A Pm45-containing single plant is selected according to the molecular marker MAG6139, the molecular marker MAG6140 and the molecular marker BARC123, and then Pm45-containing disease-resistant varieties with different genetic backgrounds can be selected and cultivated. The Pm45-containing plants can be simply, conveniently and quickly selected by using the molecular marker tightly linked with the Pm45. Due to the application of the molecular marker MAG6139, the molecular marker MAG6140 and the molecular marker BARC123, the time and the labor for selecting a disease-resistant material can be greatly saved, and the accuracy of pre-detection can be improved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to the molecular marker of wheat powdery mildew resistance gene Pm45 and its application. Background technique [0002] Wheat is the most important food crop in the world and the second largest food crop after rice in China. At present, the annual output of wheat in the world is about 620 million tons, which provides 1 / 5 of the energy needed by human beings. The production of wheat is related to the survival of human beings. Common wheat is an allohexaploid species, which contains three different chromosome sets of A, B, and D. The wild species and original species with homology or partial homology with the wheat genome contain rich gene resources. Utilizing the excellent allelic variation in wheat germplasm resources is the key factor to achieve stable and increased yield of wheat. [0003] Wheat powdery mildew is an airborne disease caused by the infection of Blumeria graminis f....

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 马正强马宏棋付必胜
Owner NANJING AGRICULTURAL UNIVERSITY
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