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Simple method for measuring average polymerization degree of xylo-oligosaccharide

A technology of average degree of polymerization and xylo-oligosaccharide, which is used in the preparation of test samples and the measurement of color/spectral characteristics, which can solve the inconvenience of macroscopic judgment of xylo-oligosaccharides and xylo-oligosaccharides, and the difficulty of factory judgment. And other issues

Inactive Publication Date: 2012-07-25
熊鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the degree of polymerization of xylo-oligosaccharides is smaller, especially the higher the concentration of xylo-oligosaccharides, the quality of xylo-oligosaccharides is better, but the average degree of polymerization corresponding to the concentration measured by this national standard cannot be judged, so it is impossible to judge macroscopically The quality of xylooligosaccharides brings inconvenience to the macroscopic judgment of xylooligosaccharides quality
In addition, this method also requires HPLC, which makes it difficult for laboratories and factories without this equipment to determine

Method used

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  • Simple method for measuring average polymerization degree of xylo-oligosaccharide
  • Simple method for measuring average polymerization degree of xylo-oligosaccharide
  • Simple method for measuring average polymerization degree of xylo-oligosaccharide

Examples

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Embodiment 1

[0022] Example 1: Take the xylooligosaccharide solution and centrifugally dilute it to 50-200 uM / L; pipette 1ml of the xylooligosaccharide solution dilution, add 25ul of 80% phenol in mass concentration, and quickly add 2.5ml of concentrated sulfuric acid to shake Evenly, let it stand for 10 minutes, then put it in a constant temperature water pool at 25-30°C for 10-20 minutes, take it out and cool it, and measure the total sugar absorbance at 480nm. Take the xylo-oligosaccharide solution and centrifugally dilute it to 5-25 mM / L, pipette 1ml of the diluted xylo-oligosaccharide solution and 1ml of distilled water into the test tube, add 3mL of DNS reagent to develop color in a boiling water bath for 5min, cool in an ice-water bath, and take it out until At room temperature, draw 200ul of sugar solution and put it into 2.5ml of distilled water in a cuvette, measure the absorbance of reducing sugar at 540nm; the total sugar and reducing sugar calculated by the standard curve are 7...

Embodiment 2

[0023] Embodiment 2: Weigh 99% xylose standard sample configuration 1mol / L, then dilute to 50-200 uM / L; pipette 1ml xylose diluent in the test tube, add 25ul of 80% mass concentration of phenol, add quickly Shake well with 2.5ml of concentrated sulfuric acid, let it stand for 10 minutes, then place it in a constant temperature pool at 25-30°C for 10-20 minutes, take it out and cool it, and measure the total sugar absorbance at 480nm. Take the xylose solution and dilute it to 5-25mM / L by centrifugation, pipette 1ml xylose dilution solution and 1ml distilled water into the test tube, add 3mL DNS reagent and develop color in boiling water bath for 5min, cool in ice water bath, take it out to room temperature, absorb 200ul of sugar Put 2.5ml of distilled water into the cuvette, and measure the absorbance of reducing sugar at 540nm; the total sugar and reducing sugar calculated by the standard curve are 11.43g / L and reducing sugar 11.26g / L respectively, and the average polymerizatio...

Embodiment 3

[0024]Embodiment 3: Take oligoxylan and prepare the solution with a concentration of 50-200 uM / L; pipette 1ml of diluent in the test tube, add 25ul of phenol with a mass concentration of 80%, quickly add 2.5ml of concentrated sulfuric acid and shake well, Let it stand for 10 minutes, then place it in a constant temperature pool at 25-30°C for 10-20 minutes, take it out and cool it, and measure its total sugar absorbance at 480nm; take oligomeric xylan to prepare a concentration of 5-25mM / L; pipette 1ml into a test tube Add 3mL of DNS reagent and 1ml of distilled water to develop color in boiling water bath for 5min, cool in ice water bath, take it out to room temperature, absorb 200ul of sugar solution and put it into 2.5ml of distilled water in a cuvette, measure the absorbance of reducing sugar at 540nm The total sugar and reducing sugar calculated by the standard curve are 8.03g / L and reducing sugar 0.74g / L respectively, and the average degree of polymerization N is 10.8; HP...

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Abstract

The invention discloses a simple method for measuring the average polymerization degree of xylo-oligosaccharide. The integral polymerization degree N of the xylo-oligosaccharide in an aqueous solution is measured through total saccharide concentration and reducing saccharide concentration in a xylo-oligosaccharide solution, thus the quality of the xylo-oligosaccharide can be macroscopically judged; when the N is equal to 1, the solution contains complete xylose; and when the N is slightly small, the component with low polymerization and high content in the xylo-oligosaccharide components in the solution can be judged. The measuring method is low in instrument requirement and simple and easy to operate; in the separation and purification process, the change of the N can reflect the change condition of each component in the solution; and the quality of the xylo-oligosaccharide is intuitively reflected by measuring the average polymerization degree serving as a macro-index.

Description

technical field [0001] The invention relates to a measuring method, in particular to a simple measuring method for the average degree of polymerization of xylooligosaccharides. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are composed of 2 to 7 D-xyloses bound by β-1, 4-xylosidic bonds, and are an important member of the functional oligosaccharide family. Its sweetness is lower than sucrose and glucose, similar to maltose, about 40% of sucrose. Xylo-oligosaccharides have good stability to pH and heat, and basically do not decompose even when heated in acidic conditions (pH=2.5~7), and are suitable for acidic beverages such as yogurt, lactic acid bacteria beverages, and carbonated beverages. Xylo-oligosaccharides are extremely difficult to be digested and absorbed, and have a high residual rate in the intestinal tract. They have excellent proliferation of bifidobacteria, and their selective utilization is higher than that of other f...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/38
Inventor 熊鹏周玉珍熊涛
Owner 熊鹏