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Simple method for measuring average polymerization degree of xylo-oligosaccharide

A technology of average degree of polymerization and xylo-oligosaccharide, which is used in the preparation of test samples and the measurement of color/spectral properties, etc. question

Inactive Publication Date: 2014-07-09
熊鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the degree of polymerization of xylo-oligosaccharides is smaller, especially the higher the concentration of xylo-oligosaccharides, the quality of xylo-oligosaccharides is better, but the average degree of polymerization corresponding to the concentration measured by this national standard cannot be judged, so it is impossible to judge macroscopically The quality of xylooligosaccharides brings inconvenience to the macroscopic judgment of xylooligosaccharides quality
In addition, this method also requires HPLC, which makes it difficult for laboratories and factories without this equipment to determine

Method used

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  • Simple method for measuring average polymerization degree of xylo-oligosaccharide
  • Simple method for measuring average polymerization degree of xylo-oligosaccharide
  • Simple method for measuring average polymerization degree of xylo-oligosaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1: Take the xylo-oligosaccharide solution and dilute it to 50-200 uM / L by centrifugation; pipette 1ml of the xylo-oligosaccharide solution diluent, add 25ul of phenol with a mass concentration of 80%, and quickly add 2.5ml of concentrated sulfuric acid to shake Evenly, let it stand for 10 minutes, then place it in a constant temperature pool of 25-30°C for 10-20 minutes, take it out to cool, and measure its total sugar absorbance at 480nm. Take the xylo-oligosaccharide solution and dilute it to 5-25 mM / L by centrifugation, pipette 1ml of the diluted xylo-oligosaccharide solution and 1ml of distilled water into the test tube, add 3mL DNS reagent in a boiling water bath to develop color for 5 minutes, cool in an ice water bath, and take it out At room temperature, draw 200ul of sugar solution into 2.5ml of distilled water in a cuvette, and measure the absorbance of reducing sugar at 540nm; the total sugar and reducing sugar calculated by the standard curve are 7.04g...

Embodiment 2

[0023] Example 2: Weigh 99% of the xylose standard sample to configure 1mol / L, and then dilute to 50-200 uM / L; pipette 1ml of xylose diluent into a test tube, add 25ul of 80% phenol by mass concentration, and quickly add Shake 2.5ml of concentrated sulfuric acid, let it stand for 10min, then place it in a constant temperature pool of 25-30℃ for 10-20min, take it out to cool, and measure its total sugar absorbance at 480nm. Take the xylose solution and dilute it to 5-25mM / L by centrifugation, pipette 1ml of xylose dilution solution and 1ml of distilled water into the test tube, add 3mLDNS reagent to the boiling water bath to develop color for 5min, cool in the ice water bath, take it out to room temperature, absorb 200ul of sugar Put the solution into 2.5ml of distilled water in a cuvette, and measure the absorbance of reducing sugar at 540nm; the total sugar and reducing sugar calculated from the standard curve are 11.43g / L and 11.26g / L respectively, and the average degree of po...

Embodiment 3

[0024] Example 3: Take oligoxylan to prepare a solution with a concentration of 50-200 uM / L; pipette 1ml of the diluent into a test tube, add 25ul of phenol with a mass concentration of 80%, and quickly add 2.5ml of concentrated sulfuric acid to shake well. Let it stand for 10 minutes, then place it in a constant temperature pool of 25-30°C for 10-20 minutes, take it out and cool it, and measure its total sugar absorbance at 480nm; take the oligoxylan configuration concentration to 5-25mM / L; pipette 1ml into the test tube Add 3mLDNS reagent to boiling water bath to develop color for 5min, cool in ice water bath, take out to room temperature, draw 200ul sugar solution into 2.5ml distilled water in a cuvette, measure the absorbance of reducing sugar at 540nm ; The total sugar and reducing sugar calculated from the standard curve are 8.03g / L, reducing sugar 0.74g / L, and the average degree of polymerization N is 10.8; HPLC detection results show that the solution is almost undetecte...

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Abstract

The invention discloses a simple method for measuring the average polymerization degree of xylo-oligosaccharide. The integral polymerization degree N of the xylo-oligosaccharide in an aqueous solution is measured through total saccharide concentration and reducing saccharide concentration in a xylo-oligosaccharide solution, thus the quality of the xylo-oligosaccharide can be macroscopically judged; when the N is equal to 1, the solution contains complete xylose; and when the N is slightly small, the component with low polymerization and high content in the xylo-oligosaccharide components in the solution can be judged. The measuring method is low in instrument requirement and simple and easy to operate; in the separation and purification process, the change of the N can reflect the change condition of each component in the solution; and the quality of the xylo-oligosaccharide is intuitively reflected by measuring the average polymerization degree serving as a macro-index.

Description

Technical field [0001] The invention relates to a measuring method, in particular to a simple method for measuring the average degree of polymerization of xylo-oligosaccharides. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are composed of 2-7 D-xyloses combined with β-1,4-xyloside bonds, and are an important member of the functional oligosaccharide family. Its sweetness is lower than that of sucrose and glucose, and is similar to maltose, about 40% of sucrose. Xylooligosaccharides have good pH and thermal stability, and they will not decompose even when heated under acidic conditions (pH=2.5 ~7). They are suitable for acidic beverages such as yogurt, lactic acid bacteria beverages and carbonated beverages. Xylooligosaccharides are extremely difficult to digest and absorb, have a high survival rate in the intestine, and have excellent bifidobacteria proliferation, and their selective utilization is higher than other functional oligosacch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/38
Inventor 熊鹏周玉珍熊涛
Owner 熊鹏