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Method for determining lactose content in cow's milk with electrochemical biosensor

A biosensor, lactose content technology, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc.

Inactive Publication Date: 2012-07-25
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography can only detect adulteration or adulteration in milk. If you want to determine adulteration or adulterated substances, you still need to use mass spectrometry or chemical methods such as kits to determine specific adulteration and adulteration. Impurities

Method used

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  • Method for determining lactose content in cow's milk with electrochemical biosensor
  • Method for determining lactose content in cow's milk with electrochemical biosensor
  • Method for determining lactose content in cow's milk with electrochemical biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Enzyme immobilization method

[0023] The change of lactose concentration is measured in the instrument because it is a dual-enzyme biosensor for measuring lactose concentration with the activity of glucose oxidase (GOD) and β-galactosidase. This instrument uses chitosan cross-linked glutaraldehyde to immobilize Enzymes have uniform stability and are less affected by external factors.

[0024] Deposit an appropriate amount of mixed solution A (mixed solution A: take 2.5 μL of glucose oxidase GOD, 2.5 mg of BSA and 5 μL of glycerol, and 40 μL of phosphate buffer solution with a pH of 7.5.) deposition (soak the glassy carbon electrode in mixed solution A After 30min in the glassy carbon electrode, the electrode ( figure 1 The two electrodes 1 is a platinum electrode, and the electrode 2 is a glassy carbon electrode) placed in glutaraldehyde vapor for 1 hour, so that it forms a network with the enzyme and chitosan. Afterwards, take an equal amount of mixed solution B...

Embodiment 2

[0053] Operation method:

[0054] The whole experimental process was carried out at constant temperature.

[0055] 1. Preparations

[0056] (1) Platinum electrode 1 and glassy carbon electrode 2 were treated according to the enzyme immobilization method described in Example 1.

[0057] (2) Prepare the blank solution (for example, distilled water, or any other substance solution) and place it in the sample cup; the outer cover of the sample cup has two ports, one port is inserted into the anode of the biosensor (platinum electrode 1), and the other port is inserted into the biosensor. The cathode of the sensor (glassy carbon electrode 2), and the two electrodes are respectively connected to the positive pole (platinum electrode 1 is connected to the positive pole) and negative pole (the glassy carbon electrode 2 is connected to the negative pole) of the microcurrent measuring instrument ( Figure 4 As shown in J4), the liquid level of the blank solution should not be lower th...

Embodiment 3

[0065] refer to image 3 , is the circuit diagram of the potentiostat of the present invention, which is used to provide power for the microcurrent measuring instrument. Operational amplifiers U1A and U1B form a differential input single-ended output amplifier circuit with a gain of 100. This circuit uses a wideband dual-channel JFET operational amplifier LF353. The sampling resistor R and other peripheral resistors are high-precision (the error is ±1%) ) metal film resistors.

[0066] refer to Figure 4 , is the circuit schematic diagram of the microcurrent measuring instrument of the present invention: ICI7135 is a 4-bit double-integral A / D conversion chip, which can convert and output ±20000 digital quantities, has BCD code output controlled by STB gate, and is very convenient to interface with the microcomputer. ICL7135 has the advantages of high precision (equivalent to 14-bit A / D conversion) and low price. Its conversion speed is related to the clock frequency. Each c...

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Abstract

The invention discloses a method for determining the lactose content in cow's milk with an electrochemical biosensor, which includes the steps: (1) treating a platinum electrode and a glassy carbon electrode by means of enzyme immobilization; (2) preparing blank solution; (3) turning on a micro-current determinator and preheating for 10min; (4) performing zero setting of the micro-current determinator according to the blank solution; (5) opening a lid of a sample cup after zero setting of the micro-current determinator is completed, and adding to-be-detected solution into the sample cup; (6) lidding the sample cup as current changes immediately after the to-be-detected solution is added, and stopping when the current is stabilized; and (7) reading a value x of the micro-current, importing the value x into a standard curve y=15.153x+1.5131 and drawing the standard curve so that the lactose content can be obtained, wherein y refers to the lactose content, and x refers to the current value. The method for determining the lactose content in the cow's milk with the electrochemical biosensor is rapid and effective, so that whether the cow's milk is adulterated or not can be distinguished, and various samples can be determined simultaneously.

Description

technical field [0001] The invention relates to the technical field of dairy product quality detection, in particular to a method for measuring lactose content in milk by using an electrochemical biosensor. Background technique [0002] The product quality and hygienic situation of our country's current dairy industry and dairy product processing industry are not optimistic. There are many problems in milk adulteration, epidemic control, hygiene management and other aspects, which make dairy food safety have major hidden dangers. Due to the lack of effective control and detection technology, dairy product quality and safety incidents have occurred frequently. The occurrence of empty shell milk powder, recycled milk, and various adulteration incidents of dairy products have seriously damaged consumers' trust in dairy product safety and greatly weakened the quality of dairy products. Market demand. Therefore, in order to ensure the quality of dairy products, various detectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327G01N27/36
Inventor 蒋珍菊林芳栋
Owner XIHUA UNIV