Induction method of radix scutellariae autotetraploid

Abstract

The invention provides an induction method of radix scutellariae autotetraploid and relates to the field of the plant multiploid induction method. Ordinary diploid radix scutellariae seeds are disinfected and cleaned for three times by 0.04 percent to 0.06 percent KMnO4 solution, and the soaking time in each time is 1 to 3 minutes; the disinfected radix scutellariae seeds are cleaned by clean water, and surface water is removed; the radix scutellariae seeds subjected to the pretreatment are uniformly distributed on a germination bed under the temperature condition being 23 to 26 DEG C, the clean water is used as germination agents until the seeds sprout from the hulls, after the seeds are taken out, surface water is removed, the seeds are transferred onto an induction bed, induction liquid with the proper consumption capable of soaking half seeds is added under the temperature condition being 25 to 29 DEG C, the induction is completed until the bud length is 2.2 to 2.5mm; the induced seeds are taken out and are flushed for 3 to 5 times by clean water, and the induction of the radix scutellariae autotetraploid is completed. The induction method is scientific and reasonable, differences caused by different time required by the proceeding of various kinds of physiological preparation during the seed germination are avoided, the damage to the seeds caused by induction liquid is greatly reduced to the maximum degree in the time aspect, the induction temperature is similar to the room temperature, the operation is easy, the difference between the induction temperature and the seeding soil temperature is small, the survival rate can be improved, in addition, the concentration of the induction liquid is very low, and the damage to the seeds caused by the induction liquid can be reduced to the maximum degree.

Description

technical field [0001] The invention relates to the field of plant polyploid induction methods, in particular to the induction method of Scutellaria baicalensis homotetraploid. Background technique [0002] Scutellaria baicalensis polyploid induction has been reported in China, mainly tissue culture method and shoot tip induction method under laboratory conditions, both methods can obtain Scutellaria baicalensis polyploid. However, because of its improper method, it has certain shortcomings. [0003] The tissue culture method to induce polyploidy is a way to induce mature cells under specific conditions, such as callus, to retransform into embryogenic meristem after dedifferentiation. Although the homozygous rate is high, it must be The detour of dedifferentiation, the process of dedifferentiation cannot be realized in production, because the operation process of tissue culture method is complicated, the cost is high, the time is long, and the polyploidy strength of Scutell...

AI Technical Summary

Problems solved by technology

This is why it is difficult to obtain homozygous tetraploids by shoot tip induction, so the agronomic traits are unstable and cannot be promoted in production