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Method for identifying resistance of sweet potatoes in seedling stage to black spot

A technology for resistance identification and black spot disease, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of result errors and other problems, achieve the effects of reducing manpower, enhancing accuracy, and reducing experimental errors

Inactive Publication Date: 2012-08-01
JIANGSU XUZHOU SWEET POTATO RES CENT
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Problems solved by technology

Qiu Ruilian et al. (1990) used the inoculation needle of potato vines in the field for identification, but due to the differences in the growth of potato vines and lignocellulose content among different varieties, it is easy to cause errors in the results.

Method used

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  • Method for identifying resistance of sweet potatoes in seedling stage to black spot

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Embodiment

[0016] Sweet potato black spot resistance identification method at the seedling stage, we carried out the black spot resistance identification test on the potato seedlings of 13 sweet potato varieties collected in the seedbed, and carried out according to the following steps:

[0017] (1) Isolation and cultivation of pathogenic bacteria: collect diseased potato pieces from the field, isolate black spot pathogenic bacteria by fungal isolation method, cultivate them on potato dextrose agar medium for 7 days, and set aside;

[0018] (2) Preparation of potato seedlings for the test: collect Huabei 52-45, Nanjing 92, Xushu 18, Zhexu 1, Xu 273, Jinong 22, Yantai 252, Xu 02-9-11, Xu 02-16 from the seedbed -17, Ning 55-1, Xu 22-1, Xu 25-2, and Shengli 100, a total of 13 sweet potato varieties (lines) of potato seedlings, cut 6 top seedlings of sweet potato with the same growth for each variety, depending on the section of the potato seedlings The length of the internodes should be 5-6...

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Abstract

The invention relates to a method for identifying resistance of crops to plant diseases and insect pests, in particular to a method for identifying resistance of sweet potatoes in a seedling stage to black spot. The method is scientific, and easy to implement, and has low requirements on experimental conditions. The method comprises the following steps of: taking shoot-tip seedlings of sweet potatoes which grow consistently in a seedling bed or a field, cutting off the lowest 2-3 leaves, cleaning by using a disinfectant fluid, putting into 1*10<4> / ml black spot spore suspension which is prepared by using sterile water in advance, culturing at room temperature of between 25 and 27DEG C for 6 days, investigating harm degrees in which experimental sweet potato seedlings are infected by the black spot, calculating each repeated average harm level, and judging the infection resistance of the experimental sweet potato seedlings to the black spot according to the harm level.

Description

technical field [0001] The invention relates to a method for identifying resistance to crop diseases, in particular to a method for identifying resistance to sweet potato black spot at seedling stage, and belongs to the field of agricultural plant protection. Background technique [0002] Sweet potato black spot is an important fungal disease that mainly damages sweet potato roots and stem bases, and occurs in all sweet potato producing areas in the world. Introduced into my country in 1937, the disease has occurred in 26 provinces, municipalities, and autonomous regions. It can be harmful in sweet potato seedbeds, fields, and storage periods. It is one of the three serious diseases in the northern potato area of ​​my country. After the potato seedlings are infected, a sunken circular or spindle-shaped small black spot will appear on the stem, and then gradually expand, and it will surround the base of the potato seedlings in the shape of black feet, and the leaves above the...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00
Inventor 孙厚俊谢逸萍赵永强徐振杨冬静张成玲
Owner JIANGSU XUZHOU SWEET POTATO RES CENT
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