Method for simultaneously preparing total caffeoylquinic acid and stevioside by taking stevia as raw material

A technology of caffeoylquinic acid and stevioside, applied in chemical instruments and methods, preparation of carboxylic acid esters, preparation of sugar derivatives, etc., can solve problems such as environmental pollution and resource waste, reduce environmental pollution and improve comprehensive benefits , the effect of shortening the production cycle

Active Publication Date: 2012-08-01
巴州正达绿源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The impurity removal process such as chemical precipitant calcium salt and iron salt used in the production process will precipitate the total caffeoylquinic acids in stevia, resulting in a serious waste of resources, and these precipitated total caffeoylquinic acids After the waste residue is discharged into the environment, it will pollute the environment

Method used

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  • Method for simultaneously preparing total caffeoylquinic acid and stevioside by taking stevia as raw material
  • Method for simultaneously preparing total caffeoylquinic acid and stevioside by taking stevia as raw material
  • Method for simultaneously preparing total caffeoylquinic acid and stevioside by taking stevia as raw material

Examples

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example 1

[0035] Example 1: Take 1 kg of stevia leaf powder, add 20 L of water or deionized water, adjust the pH to 3.0, and boil for 5 minutes to extract. Filter and concentrate after extraction. The concentrated solution was extracted with ethyl acetate; the ethyl acetate extract in the upper layer was concentrated, then adsorbed with NKA-9 macroporous resin column, and eluted with deionized water and ethanol with a volume concentration of 60% in sequence to collect the total coffee containing The eluate of dicaffeoylquinic acid in acylquinic acid was concentrated and dried to obtain 15.90 g of dicaffeoylquinic acid with a content of 80.0%. The extracted extract in the lower layer is adsorbed on the AB-8 macroporous resin column, eluted with deionized water and ethanol solution in turn, and the eluate containing monocaffeoylquinic acid and stevioside is collected, concentrated and dried to obtain the content 3.78g of 78.56% monocaffeoylquinic acid; after the eluate of stevioside is c...

example 2

[0036] Example 2: Take 1 kg of Stevia rebaudiana leaf powder, add 40 L of 70% ethanol solution, adjust the pH to 3.5, and perform ultrasonic extraction. Filter and concentrate after extraction. The concentrated solution was extracted with ethyl acetate; the ethyl acetate extract in the previous step was concentrated, then adsorbed with HPD500 macroporous resin column, eluted with deionized water and ethanol with a volume concentration of 70% in sequence, and collected the dicaffeoylquinone containing The eluate of nicotinic acid was concentrated and dried to obtain 17.40 g of dicaffeoylquinic acid with a content of 92.45%. The extracted extract was adsorbed on HPD450 macroporous resin column, eluted with deionized water and ethanol solution successively, and the eluate containing monocaffeoyl quinic acid was collected, concentrated and dried to obtain monocaffeoyl quinic acid with a content of 90.32%. Quinic acid 4.72g. After the eluate of stevioside is concentrated, calcium...

example 3

[0037] Example 3: Get the stevia leaf powder of 1kg, add 80L of 50% methanol solution, adjust the pH to 4.0, ultrasonic extraction (see the total caffeoylquinic acid content in the extract figure 1 , for total stevia content see figure 2 ). Filter and concentrate after extraction. The concentrated solution was extracted with ethyl acetate; the ethyl acetate extract in the previous step was concentrated, then adsorbed with NKA-II macroporous resin column, eluted with deionized water and ethanol with a volume concentration of 50% in sequence, and collected the The eluate of acylquinic acid was concentrated and dried to obtain 16.85 g of dicaffeoylquinic acid with a content of 81.50%. The extracted extract was adsorbed on a D201 macroporous resin column, eluted with deionized water and ethanol solution in turn, and the eluate containing monocaffeoyl quinic acid was collected, concentrated and dried to obtain monocaffeoyl quinic acid with a content of 83.12%. 4.56g of quinic a...

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Abstract

The invention relates to a method for simultaneously preparing total caffeoylquinic acid and stevioside by taking stevia as a raw material. The method comprises the following steps that: the stevia is extracted with water or an organic solvent under the action of heating, microwave or ultrasound-assisting, and a stevioside and caffeoylquinic acid extraction liquid is obtained after solid-liquid separation, and then is subjected to extraction separation; and finally, a pure product of total steviol glycoside and total caffeoylquinic acid is obtained after purification. On the basis of the traditional stevia production process, a total caffeoylquinic acid extraction process is added, so that the two types of products, i.e., the stevioside and the total caffeoylquinic acid, can be simultaneously obtained by taking the stevia as the raw material, the raw material source of the total caffeoylquinic acid substance is broadened, and the two types of high-value-added products can be simultaneously produced by the same raw material, and thus the production costs of the two types of products can be greatly reduced, the waste of resources and the environmental pollution can be reduced, the requirements of the sustainable development strategy can be met, and the application prospect is good.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to a method for simultaneously extracting steviosides and caffeoylquinic acids with stevia. Stevia is leached with hot water, ultrasonic wave or microwave, and the total caffeoylquinic acid and stevioside are extracted at the same time, separated by organic solvent extraction and separated and purified by resin, so as to obtain high-purity stevia Sugar and caffeoylquinic acid products, the residue after extraction is used to produce feed or organic fertilizers. Background technique [0002] Stevia rebaudinan Betroni (Stevia rebaudinan Betroni) is a perennial herb of the Asteraceae Stevia genus, native to the Amambay and Mbaxacayu mountains in Paraguay. Stevia, as a new sugar source plant, has attracted attention during the Second World War. Introduced from Brazil in 1970, Japan began domestication, cultivation, sugar production, and experiments on toxicology and food testing. my cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08C07C69/732C07C67/58A23K1/14C05F5/00C05F11/08
CPCY02A40/20Y02E50/30Y02W30/40
Inventor 陈育如张怡斐马迎迎王建芬刘润薇刘珺
Owner 巴州正达绿源生物科技有限公司
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