Shotgun shooting type artificial infection method of coral

A kind of coral and artificial technology, applied in animal husbandry and other fields, can solve the problems of difficult coral diseases, bacterial pathogens, etc., and achieve the effect of reducing work intensity

Inactive Publication Date: 2012-08-15
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And because polyps and zooxanthellae are relatively small individuals, it is impossible to directly isolate pathogens from their bodies. Therefore, no matter whether healthy coral tissue or diseased coral tissue is used a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A shotgun targeting type coral artificial infection method is characterized in that:

[0027] Determining the bacterial pathogen of branching corals involves the following steps:

[0028] A. Sample collection, processing and pathogen isolation, purification and expanded culture

[0029] Collect 0.5g of coral tissue at the diseased part in the ultra-clean workbench, grind it into powder with a mortar, transfer the powder into a centrifuge tube with 1ml of sterilized seawater, blow it with a pipette for 5 minutes, and let it stand at room temperature for 5 minutes , absorb 100μl of the supernatant and spread it on a solid 2216E seawater medium or TCBS medium plate; incubate at room temperature for 24 hours, pick multiple single colonies of different shapes on the plate, and inoculate them in 1ml of liquid 2216E respectively Expand the culture in the cryopreservation tube of seawater culture medium, so that the concentration of the bacterial solution reaches 10 9 CFU / ml;...

Embodiment 2

[0039] A shotgun targeting type coral artificial infection method is characterized in that:

[0040] Determining the bacterial pathogen of branching corals involves the following steps:

[0041] A. Sample collection, processing and pathogen isolation, purification and expanded culture

[0042]Collect 0.7g of coral tissue at the diseased part in the ultra-clean workbench, grind it into powder with a mortar, transfer the powder into a centrifuge tube with 1ml of sterilized seawater, blow it with a pipette for 5 minutes, and let it stand at room temperature for 5 minutes , absorb 100μl of the supernatant and spread it on a solid 2216E seawater medium or TCBS medium plate; incubate at room temperature for 35 hours, pick multiple single colonies of different shapes on the plate, and inoculate them in 1ml of liquid 2216E respectively Expand the culture in the cryopreservation tube of seawater culture medium, so that the concentration of the bacterial solution reaches 10 9 C...

Embodiment 3

[0052] A shotgun targeting type coral artificial infection method is characterized in that:

[0053] Determining the bacterial pathogen of branching corals involves the following steps:

[0054] A. Sample collection, processing and pathogen isolation, purification and expanded culture

[0055] Collect 1.0g of coral tissue from the diseased part in the ultra-clean workbench, grind it into powder with a mortar, transfer the powder into a centrifuge tube with 1ml of sterilized seawater, blow it with a pipette for 5 minutes, and let it stand at room temperature for 5 minutes , absorb 100μl of the supernatant and spread it on a solid 2216E seawater medium or TCBS medium plate; incubate at room temperature for 48 hours, pick multiple single colonies of different shapes on the plate according to the different forms of the colony, and inoculate them in 1ml of liquid 2216E respectively Expand the culture in the cryopreservation tube of seawater culture medium, so that the conce...

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PUM

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Abstract

The invention relates to an artificial infection method of a tree coral. The technical scheme is described as follows: firstly, randomly dividing strains obtained and separated from a diseased coral into a plurality of test groups; simultaneously carrying out artificial infection on healthy corals by utilizing the parallel groups of mixed bacterium liquid; respectively carrying out artificial infection on the healthy corals by utilizing the strains of the test groups capable of inducing the healthy corals to be diseased; and finally, confirming the bacterium pathogens capable of inducing the healthy corals to be diseased, therefore, processes of rapidly and accurately separating and identifying the coral bacterium pathogens are realized. The method disclosed by the invention has the beneficial effects that: aiming at coral bacterium diseases, the rapid separation and confirmation of pathogenic bacteria are realized; and compared with a traditional method, the method is capable of greatly reducing the working intensity of coral bacterial disease epidemiological investigation.

Description

[0001] technical field [0002] The invention relates to a shotgun-targeting coral artificial infection method. Background technique [0003] Porites flat branch ( Porites andrewsi Vaughan) and other branched corals are the main dominant species of reef-building stony corals in tropical seas, and these corals often suffer from various diseases such as bleaching. In recent years, frequent coral diseases have led to serious degradation of coral reef ecosystems, affecting the balance of global coral reef ecosystems, and have received great attention. Many important international scholars have suggested establishing methods that can effectively identify coral pathogens as soon as possible. However, since coral is a type of open lower eukaryotic animal living in the marine environment, the polyps and unicellular zooxanthellae live in symbiosis. The exchange of substances, resulting in the possibility of pathogenic bacteria in the water infecting polyps or zooxanthellae. A...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 谢珍玉周永灿胡超群
Owner HAINAN UNIVERSITY
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