Shotgun shooting type artificial infection method of coral

Abstract

The invention relates to an artificial infection method of a tree coral. The technical scheme is described as follows: firstly, randomly dividing strains obtained and separated from a diseased coral into a plurality of test groups; simultaneously carrying out artificial infection on healthy corals by utilizing the parallel groups of mixed bacterium liquid; respectively carrying out artificial infection on the healthy corals by utilizing the strains of the test groups capable of inducing the healthy corals to be diseased; and finally, confirming the bacterium pathogens capable of inducing the healthy corals to be diseased, therefore, processes of rapidly and accurately separating and identifying the coral bacterium pathogens are realized. The method disclosed by the invention has the beneficial effects that: aiming at coral bacterium diseases, the rapid separation and confirmation of pathogenic bacteria are realized; and compared with a traditional method, the method is capable of greatly reducing the working intensity of coral bacterial disease epidemiological investigation.

Description

[0001] technical field [0002] The invention relates to a shotgun-targeting coral artificial infection method. Background technique [0003] Porites flat branch ( Porites andrewsi Vaughan) and other branched corals are the main dominant species of reef-building stony corals in tropical seas, and these corals often suffer from various diseases such as bleaching. In recent years, frequent coral diseases have led to serious degradation of coral reef ecosystems, affecting the balance of global coral reef ecosystems, and have received great attention. Many important international scholars have suggested establishing methods that can effectively identify coral pathogens as soon as possible. However, since coral is a type of open lower eukaryotic animal living in the marine environment, the polyps and unicellular zooxanthellae live in symbiosis. The exchange of substances, resulting in the possibility of pathogenic bacteria in the water infecting polyps or zooxanthellae. A...

AI Technical Summary

Problems solved by technology

And because polyps and zooxanthellae are relatively small individuals, it is impossible to directly isolate pathogens from their bodies. Therefore, no matter whether healthy coral tissue or diseased coral tissue is used as a sample, a variety of different colony forms can be obtained at the same time. bacteria, it is difficult to determine the bacterial pathogens of coral diseases by artificial infection methods based on Cook's principle