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Method for identifying spirulina strains with favorable production traits

A technology for producing traits and spirulina, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of high cost, heavy workload, cumbersome procedures, etc., and achieve the effect of low cost

Inactive Publication Date: 2012-08-15
ZHEJIANG UNIV
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Problems solved by technology

Although this method is practical and effective, the procedure is cumbersome, the workload is heavy, the cycle is long, and the cost is high.

Method used

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  • Method for identifying spirulina strains with favorable production traits
  • Method for identifying spirulina strains with favorable production traits
  • Method for identifying spirulina strains with favorable production traits

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Embodiment Construction

[0026] The inventor found in the long-term molecular genetics research of Spirulina that Spirulina, like other cyanobacteria such as Anabaena and Anabaena, has a special highly palindromic sequence in the genome that varies due to varieties (lines) (highly iterated palindromic sequence, HIPS) [Microbiology, 1998, 144: 2791-2801]. The present invention establishes a method that uses the specific sequence HIPS-TC: 5'-GCGATCGCTC-3' in HIPS as a primer and combines PCR (polymerase chain reaction) technology to identify the excellent production properties of spirulina and whether it can be applied to large-scale breeding production. new method.

[0027] We used the specific sequence HIPS-TC in HIPS: 5'-GCGATCGCTC-3' as a primer to 14 strains of Spirulina ZJU0101, ZJU0102, ZJU0103, ZJU0104, ZJU0105, ZJU0106, ZJU0107, ZJU0108, ZJU0109 / RH, ZJU0110, ZJU01114, ZJU0010 The DNA polymorphisms of ZJU0115 and ZJU0116 were amplified, and by electrophoresis and cluster analysis, it was found ...

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Abstract

The invention relates to the development and application technology of spirulina, and aims to provide a method for identifying spirulina strains with favorable production traits. The method provided by the invention comprises the steps of enhancing genomic DNA (deoxyribose nucleic acid) of a candidate strain by a special primer HIPS-TC, and establishing a dendrogram of the spirulina strains by adopting NTSYpc2.1 software after electrophoretic analysis. If the candidate strains have 1130bp, 620bp, 580bp and 540bp special DNA electrophoretic bands, and can be compounded with the existing strain with favorable production traits, the production traits of the candidate strains are favorable, and are suitable for large scale cultivation and production. If the candidate strains have 1190bp, 780bp, 580bp,520bp, and 470bp special DNA electrophoretic bands, and can be compounded with the existing strain with poor production traits, the production traits of the candidate strains are poor, and are not suitable for large scale cultivation and production. Compared with the common method, the method for identifying spirulina strains with favorable production traits in the invention has the advantages of simpleness, fast speed, efficiency and low cost, and is suitable for large-scale and high throughput screening.

Description

technical field [0001] The invention belongs to the technology for the development and application of spirulina, in particular to a method for identifying strains with excellent production properties of spirulina. Background technique [0002] Spirulina is a kind of prokaryotic filamentous microalgae with photosynthetic oxygen release and regular spiral, which is a genus of Cyanophyta, Oscillatoriales and Oscillatoriaceae [Wuhan Botany Research, 1997, 15(4):369-374]. Because it is rich in high-quality protein and a variety of biologically active substances, it has attracted great attention at home and abroad, and has formed a huge Spirulina industry on the basis of a large number of researches. It has become the world's largest research and development scale and the most extensive application prospects. microalgae. It is worth pointing out that numerous experimental studies and long-term production practices have shown that different varieties (lines) of Spirulina have dif...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 汪志平邵斌刘新颖于金鑫蓝瑾瑾王景梅陈子元
Owner ZHEJIANG UNIV
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