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Detection method of Wuweiganlu preparation

A detection method, the technology of nectar, applied in the direction of measuring devices, preparation of test samples, instruments, etc., can solve the problems of no medicinal materials, no content determination, and difficulty in displaying microscopic identification to the characteristics of preparations, etc.

Active Publication Date: 2014-03-26
TIBET QIZHENG TIBETAN MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] There are the following problems in the original detection method of Wuwei Ganlu Yaoyu Decoction: ①There is no medicinal material identified in the microscopic identification standard, so it is difficult to show the characteristics of the preparation by microscopic identification; ②There is only one ephedrine TLC identification method for TLC identification; ③No content determination
[0019] And can effectively control arthralgia (rheumatoid arthritis, rheumatoid arthritis), gout, the Tibetan medicine preparation product quality of hemiplegia at present, can operate the detection method easily again, also do not report

Method used

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  • Detection method of Wuweiganlu preparation
  • Detection method of Wuweiganlu preparation
  • Detection method of Wuweiganlu preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0085] Experimental example 1: Microscopic identification of juniper and water cypress branches

[0086] (1) Instrument

[0087] Microscopes, cameras, glass slides, coverslips, electronic balances, pipettes, filter paper, sonicators.

[0088] (2) Reagents

[0089] Chloral Hydrate, Glycerin, Distilled Water, Acetic Acid.

[0090] (3) Inspection method:

[0091] Selection of permeabilization solvent: Use chloral hydrate test solution, acetic acid glycerin test solution, dilute glycerin and other solutions as permeabilization solvents;

[0092] Selection of permeabilization method: adopt traditional heating permeabilization method and oven heating method respectively;

[0093] The above permeabilization solvents and permeabilization methods were respectively selected for testing, and the results are shown in Table 1 below:

[0094] Table 1

[0095]

[0096] Repeated tests under the above conditions finally determined the specific microscopic identification method of ju...

experiment example 2

[0098] Experimental example 2: TLC identification of rhododendron

[0099] (1) Instrument

[0100] Mortar, electronic scale, measuring cylinder, flat bottom flask, condenser tube, rubber tube, water bath, ultrasonic extractor, funnel, filter paper, evaporating dish, separatory funnel, absorbent cotton, spotter, silica gel GF254 plate, chromatography cylinder, ultraviolet spot analyzer.

[0101] (2) Control medicinal materials

[0102] Rhododendron as reference medicinal material (batch number: 121394-200401) was purchased from China Institute for the Identification of Pharmaceutical and Biological Products.

[0103] (3) Reagents

[0104] Ethanol, ether, methanol, toluene, ethyl acetate, formic acid, distilled water, acetone.

[0105] (4) Inspection method:

[0106] Selection of extraction solvent: use methanol, ethanol, and acetone as extraction solvents respectively;

[0107] Selection of extraction methods: ultrasonic and heating reflux respectively;

[0108] The ch...

experiment example 3

[0114] Experimental example 3: TLC identification of juniper

[0115] (1) Instrument

[0116] Mortar, electronic scale, measuring cylinder, Erlenmeyer flask, flat-bottomed flask, condenser tube, rubber tube, water bath, ultrasonic extractor, funnel, filter paper, sample spotter, silica gel G plate, chromatography cylinder, UV spotting analyzer.

[0117] (2) Control medicinal materials

[0118] Juniper control medicinal material (batch number: 121485-200503) was purchased from China National Institute of Pharmaceutical and Biological Products.

[0119] (3) Reagents

[0120] Absolute ethanol, methanol, ethanol, chloroform, ammonia, distilled water.

[0121] (4) Inspection method:

[0122] Selection of extraction solvent: absolute ethanol, methanol, and ethanol are used as extraction solvents;

[0123] Selection of extraction methods: ultrasonic and heating reflux respectively;

[0124] The choice of developer: respectively use chloroform-methanol-concentrated ammonia tes...

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Abstract

The invention provides a detection method of a Wuweiganlu preparation. The method comprises the following steps of: performing microscopic identification of the microscopic characteristics of juniperus formosana and myricaria in the Wuweiganlu preparation; performing thin-layer chromatography identification of rhododendron anthopogonoide, juniperus formosana and artemisia sieversiana in the Wuweiganlu preparation; and measuring the content of ephedrine hydrochloride and pseudoephedrine hydrochloride in ephedra and the content of artemisetin in artemisia sieversiana in the preparation by a high performance liquid chromatography. The detection method provided by the invention has the advantages of good reproducibility and stability, high precision, strong specificity, clear spot color, high separation degree, accurate content and the like, and is simple to operate; and by creating a reliable quality detection method with strong specificity, the quality of the Wuweiganlu preparation can be effectively controlled so that the quality of the Wuweiganlu preparation is stable, safe and controllable.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of Wuwei nectar preparation, which belongs to the technical field of detection of traditional Chinese medicine. Background technique [0002] The detection method of Wuwei Ganlu Medicinal Yutang Powder is recorded in the first volume of the standard issued by the Ministry of Health of the People's Republic of China, page 269 (WS3-BC-0266-95). The five-flavored nectar medicinal bath powder made of ephedra, juniper, water cypress branch, big seed wormwood, and rhododendron rhododendron has the functions of sweating, anti-inflammatory, pain relief, drying yellow water, promoting blood circulation and dredging collaterals. For arthralgia (rheumatoid arthritis, rheumatoid arthritis), gout, hemiplegia, etc. [0003] [Prescription 1 Juniper 100g, Rhododendron 100g, Artemisia grandis 100g, Ephedra 100g, Cypress branch 100g. [0004] [Preparation m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/90G01N30/06G01N1/38G01N1/44
Inventor 刘鸿雁陈丽娟张国霞
Owner TIBET QIZHENG TIBETAN MEDICINE
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