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Reversible current gel electrophoresis device for separating biological macromolecules

A technology of biomolecules and electrophoresis devices, which is applied in the determination/testing of microorganisms, separation methods, measurement devices, etc., and can solve problems such as difficult access

Inactive Publication Date: 2015-07-08
OREGON HEALTH & SCI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the target biomolecules will be dispersed in the cross-linked gel and not easily accessible

Method used

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  • Reversible current gel electrophoresis device for separating biological macromolecules
  • Reversible current gel electrophoresis device for separating biological macromolecules
  • Reversible current gel electrophoresis device for separating biological macromolecules

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Embodiment Construction

[0041] I. Terminology

[0042]Unless otherwise stated, technical terms are used according to conventional usage. Definitions of commonly used terms in molecular biology can be from Benjamin Lewin's "Gene VII" (Genes VII, published by Oxford University Press, 2000 (ISBN 019879276X)); "Encyclopedia of Molecular Biology" by Kendrew et al. (The Encyclopedia of Molecular Biology, published by Blackwell Press, 1994 (ISBN 0632021829)); Molecular Biology and Biotechnology: A Comprehensive Reference Book by Robert A. Meyers (Editor) (Molecular Biology and Biotechnology: a Comprehensive Desk Reference, Wiley, John & Sons, 1995 (ISBN 0471186341)) and George P. Rédei, Encyclopedic Dictionary of Genetics, Genomics, and Proteomics 2nd Edition , 2003 (ISBN: 0-471-26821-6)).

[0043] The following explanations of terms and methods are used to better describe the present invention and guide those of ordinary skill in the art to implement the present invention. The singular forms "a", "an" a...

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Abstract

Cassette bodies for use with electrophoresis apparatus can be formed of a single piece of molded or machined plastic. Such cassette bodies can include a plurality of channels that pass through the cassette body, from a proximal end to a distal end. Such channels can be defined by upper and lower chambers. The upper chambers can be in fluid communication with a first buffer pool through a semi-permeable membrane, and the lower chambers can be in fluid communication with a second buffer pool. An electric current can be passed through the first and second buffer pools, and then reversed, to perform an electrophoresis operation that can separate a biomolecule of interest from free probes, and provide for convenient collection of said biomolecule of interest.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to U.S. Provisional Patent Application No. 61 / 239,017, entitled "Reversible Current Gel Electrophoresis Apparatus for the Separation of Biomacromolecules," filed September 1, 2009, the disclosure of which is hereby Incorporated herein by reference. technical field [0003] The invention relates to a device for electrophoretic purification of biological macromolecules and a method for using the device. Background technique [0004] Electrophoresis is a method for separating and analyzing biological macromolecules including proteins, DNA, RNA or their complexes. Various electrophoretic techniques have been developed including capillary electrophoresis, gel electrophoresis, paper electrophoresis, and immunoelectrophoresis. [0005] The most common electrophoresis method is gel electrophoresis. In gel electrophoresis, a gel is made using compounds such as agarose or polyac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447C12Q1/68G01N27/416G01N33/50
CPCG01N33/6803B01D57/02G01N27/44773G01N27/44756
Inventor 叶正W·马瑟斯E·布莱特曼
Owner OREGON HEALTH & SCI UNIV