Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof

The technology of Paecilomyces lilacinus and Verticillium pachysporum is applied in the field of compositions for killing nematodes by using nematode parasites and the field of preparation thereof, which can solve the problem of ineffectiveness of pesticide varieties, unavailability, long development cycle of new pesticides, etc. problems, to overcome the long development cycle of new drugs, promote growth, and have good nematicide effects.

Active Publication Date: 2014-02-19
GUANGDONG ZHENGE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to long-term, single use or abuse of pesticides in agricultural production, pests have developed resistance to pesticides, and even developed cross-resistance and multi-resistance, making many pesticide varieties useless
However, the research and development cycle of new pesticides is long, and they are often not available, which is a major obstacle to agricultural production.

Method used

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  • Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof
  • Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof
  • Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof

Examples

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Effect test

preparation example Construction

[0039] A preparation method of a nematicidal composition containing Verticillium pachyspora and Paecilomyces lilacinus combined, comprising the following steps:

[0040] (1) Disperse the mother powder of Verticillium pachyspora and Paecilomyces lilacinus uniformly in a homogenizer equipped with solvents and additives to obtain a mixture of Verticillium pachyspora and Paecilomyces lilacinus with uniform viscosity bacterial liquid;

[0041] (2) Transfer the prepared mixed bacterial liquid to a high-level container;

[0042] (3) Put the carrier into the mixer;

[0043] (4) Start the stirrer, open the discharge valve of the high-level container, and add the mixed bacterial liquid until the bacterial liquid is added;

[0044] (5) Check the effect of the adsorption method for granulation, and check the technical indicators of the product.

Embodiment 1

[0045] Example 1 Study on the synergistic effect

[0046] In order to overcome and delay the resistance of pests and prolong the service life of old pesticide varieties, we used Verticillium pachysporum and Paecilomyces lilacinus to combine pesticides to study the synergistic effect on R. incognita.

[0047] Pick tomato roots artificially inoculated with Nematode incognita, crush them with a tissue masher, pass through 200-mesh, 325-mesh, and 500-mesh nematode standard sieves in turn, rinse the eggs on the 500-mesh sieve with sterile water, and prepare eggs Suspension (100 eggs / mL). Another part of the eggs was incubated in sterile water, and the live larvae (2nd instar larvae) hatched on the second day were used for testing. The fresh Paecilomyces lilacinus fermentation broth was prepared into a mother liquor of a certain concentration, and then mixed with Verticillium pachysporum according to (6:1, 4:1, 2:1, 1:1 and 1:2) for the test. Nematode larvae or egg suspension (100...

Embodiment 2

[0048] Preparation of the composition of Example 2

[0049] The invention provides a case of a nematicidal granule containing a combination of Verticillium pachyspora and Paecilomyces lilace and a preparation method thereof:

[0050] The components and contents in the granules can be determined according to 1) 50 grams of live mother powder of Verticillium pachysporum 2.5 billion per gram, 50 grams of mother powder containing 20 billion per gram of Paecilomyces lilacinus, stabilizer soybean oil 50 grams grams, 100 grams of solvent mineral oil, 750 grams of carrier kaolin. 2) 50 grams of live mother powder containing 2.5 billion / gram Verticillium pachyspora, 50 grams of mother powder containing 20 billion / gram Paecilomyces lilacinus, 50 grams of methyl solvate, 50 grams of stabilizer castor oil, carrier Kaolin about 800 grams.

[0051] The Verticillium pachyspora and Paecilomyces lilacinacea nematicidal granules are prepared through the following steps: (1) The mother powder ...

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Abstract

The invention relates to a nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and a preparation method thereof. The nematicidal composition consists of the verticillium chlamydosporium and the Paecilomyces lilacinus, and also comprises a solvent, an aid and a stabilizer. The nematicidal composition comprises the following components in percentage by weight: 0.1 to 10 percent of verticillium chlamydosporium, 0.1 to 20 percent of Paecilomyces lilacinus, 1 to 15 percent of stabilizer, 5 to 15 percent of solvent, 1 to 10 percent of aid and 60 to 90 percent of carrier. The verticillium chlamydosporium and the Paecilomyces lilacinus are combined to achieve a synergistic effect, and a nematicidal effect is better; the verticillium chlamydosporium and the Paecilomyces lilacinus are bacterial parasites for nematode, a biocontrol technique is used, and is environment-friendly and safe, and problems that the nematode has resistance to pesticides, a development cycle for a new pesticide is long and the like are solved; and the verticillium chlamydosporium and the Paecilomyces lilacinus can promote the growth of plants to achieve effects of increasing production and value.

Description

technical field [0001] The present invention relates to a nematicidal composition, in particular to a nematicidal composition using nematode parasites and a preparation method thereof. Background technique [0002] Root-knot nematodes mainly damage the roots of various vegetables, showing that the lateral roots and fibrous roots are more than normal, and form spherical or conical white nodules of varying sizes on the fibrous roots of young roots, and some are in the shape of rosary. The aboveground growth of the infected plants is short, slow, and the leaf color is abnormal, the results are few, the yield is low, and even the plants die prematurely. At present, the nematodes known to damage vegetables mainly include Toxocarpus tallis, peanut root-knot nematodes, northern root-knot nematodes and southern root-knot nematodes. Nematodes have a wide range of hosts, often injuring more than 30 kinds of vegetables such as melons, solanaceous fruits, beans and radishes, carrots, l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N25/08A01P5/00
CPCY02A40/10
Inventor 赵勇王泊理高英超张天成
Owner GUANGDONG ZHENGE BIOLOGICAL TECH CO LTD
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