The invention provides an automatic doffing system for twisting production. The system comprises a twisting machine main machine, a guiding and positioning device, an upper yarn lifting device, a hanging and conveying device, a lower yarn lifting device and a transferring device which are sequentially arranged according to the passing sequence of yarn rolls, and further comprises a master controldevice electrically connected with the above devices, so that the automatic doffing operation process is achieved. According to the system, wound yarn coils are automatically unloaded from the twisting machine main machine, stacked in order and directly stored in a warehouse, manual operation is omitted, the working efficiency is greatly improved, meanwhile, possible yarn coil damage in the manualdoffing process is avoided, and the quality and consistency of products are guaranteed; the length of the hanging and conveying device can be automatically set, so that the hanging and conveying device is matched with a plurality of twisting machine main machines and a transfer device at the same time, the doffing work efficiency is further improved, the automation degree is improved, and equipment in a plant can be simplified.