Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing avian influenza vaccine by using cells cultured by spherical microcarrier bioreactor

A bioreactor and cell culture technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as limited production output, and achieve the effect of shortening the production cycle

Inactive Publication Date: 2012-09-12
ZHAOQING DAHUANONG BIOLOGIC PHARMA +1
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amount of virus produced only by animal cell culture is limited, so the application of vector culture technology to the production of avian influenza vaccine will undoubtedly not only maintain the advantages of cell culture but also overcome the shortcomings of its limited production output.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing avian influenza vaccine by using cells cultured by spherical microcarrier bioreactor
  • Method for producing avian influenza vaccine by using cells cultured by spherical microcarrier bioreactor
  • Method for producing avian influenza vaccine by using cells cultured by spherical microcarrier bioreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: microcarrier bioreactor suspension culture breeding H5 subtype avian influenza inactivated vaccine

[0027] 1: Select the type of cell MDCK required for the introduction of the vector;

[0028] 2: The method of spinner bottle propagating cell passage: first resuscitate the seed cell MDCK selected in step 1 into a T225 cell flask, grow for about 48 hours, and passage according to the ratio of 1:4; then put 4 full-celled T225 cells The cells in the bottle were transferred to a 15L spinner bottle for culture; the cells were subcultured at a ratio of 1:3 for about 48 hours; the medium used in this process was MEM, the serum was fetal bovine serum, and the amount used was 7%;

[0029] 3: Harvest the cells in the spinner bottle: wash the cells obtained in step 2 with PBS 2-3 times, then add 125ml of 0.25% trypsin-EDTA solution to digest and collect the cells;

[0030] 4: After counting the harvested cells, the selected microcarrier is Cytodex I, and the cells a...

Embodiment 2

[0046] Embodiment 2: Microcarrier suspension culture propagation H9 subtype avian influenza virus

[0047] 1: Select the type of cell MDCK required for the introduction of the vector;

[0048] 2: The method of spinner bottle propagating cell passage: first resuscitate the seed cell MDCK selected in step 1 into a T225 cell flask, grow for about 48 hours, and passage according to the ratio of 1:4; then put 4 full-celled T225 cells The cells in the bottle were transferred to a 15L spinner bottle for culture; the cells were subcultured at a ratio of 1:4 for about 48 hours; the medium used in this process was MEM, the serum was fetal bovine serum, and the amount used was 7-10 %;

[0049] 3: Harvest the cells in the spinner bottle: wash the cells obtained in step 2 with PBS 2-3 times, then add 125ml of 0.25% trypsin-EDTA solution to digest and collect the cells;

[0050] 4: After the harvested cells are counted, the selected microcarrier is hyclone, and the cells are inoculated ac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for producing an avian influenza vaccine by using cells cultured by a spherical microcarrier bioreactor. The method comprises the following steps of: selecting seed cells; passaging cells reproduced in a spinner bottle; harvesting the cells in the spinner bottle; inoculating the cells; culturing the inoculated cells in the bioreactor; inoculating avian influenza virus; reproducing the avian influenza virus; collecting avian influenza virus liquid; inactivating the avian influenza virus liquid; preparing an avian influenza cell vaccine; and immunizing the avian influenza cell vaccine. By the method, the defects that the avian influenza vaccine produced by the traditional process is long in production period, large in inter-batch difference, high in endotoxin content and influenced by the supply of embryo eggs, and a tablet carrier is difficult to amplify are overcome; and the prepared avian influenza vaccine product is small in inter-batch difference, free from bacterial pollution, low in endotoxin content and formaldehyde content, stable in quality and short in the production period, and the tablet carrier is easy to amplify.

Description

technical field [0001] The invention relates to the technical field of bird flu vaccines, in particular to a method for producing bird flu vaccines by using spherical microcarrier bioreactors to cultivate cells. Background technique [0002] In the past, our country has been producing influenza vaccines through chicken embryo culture. Since the production of influenza vaccine from chicken eggs requires the consumption of a large number of chicken embryos, the production cycle is long, it is easy to be polluted, and it is not easy to control, and each fertilized egg can only be injected with one virus strain at a time, so this production method is very inefficient and is not conducive to large-scale flu outbreak. [0003] Using mammalian cells as the matrix to prepare vaccines has the advantages of no exogenous factor contamination, easy large-scale production, and better stability of virus antigens. However, the amount of virus produced only by animal cell culture is limit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/145A61P31/14C12N7/00C12N7/06C12R1/93
Inventor 陈瑞爱徐家华施维松张冬霞汤钦
Owner ZHAOQING DAHUANONG BIOLOGIC PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com