Sterilization method of inorganic culture solution for different microalgae fermentation

Abstract

The invention discloses a sterilization method of an inorganic culture solution for different microalgae fermentation, which belongs to the technical field of sterilization. The sterilization method comprises the steps of: adding sodium hypochlorite solid in the inorganic culture solution for the different microalgae fermentation, uniformly stirring the solution, standing, introducing sterile air into the solution to perform oxygen exposure and chloride dispersion, and then adding sodium thiosulfate in the solution to neutralize residual chloride; and adding the inorganic culture solution for the different microalgae fermentation and carbon and nitrogen nutrition sources in a sterilization tank for mother solution of the inorganic culture solution for the different microalgae fermentationto form the mother solution, performing routine sterile disinfection on the mother solution, sucking the inorganic culture solution for the different microalgae fermentation in a different microalgae fermentation device while adding the mother solution in the different microalgae fermentation device, and increasing the temperature in a main fermentation tank by excess heat of the mother solution, thereby finishing the process of sterilization and temperature rising of the inorganic culture solution for the different microalgae fermentation and then allowing the inoculation of algae seedlings, wherein the sterilization tank for the mother solution of the inorganic culture solution for the different microalgae fermentation is arranged at the external upper part of the different microalgae fermentation device.

Description

Technical field: [0001] The invention belongs to the technical field of disinfection and sterilization, and relates to a disinfection and sterilization method for inorganic culture fluid, in particular to a disinfection and sterilization method for microalgae heterotrophic fermentation inorganic culture fluid. Background technique: [0002] Schizochytrium limacinum, also known as Schizochytrium limacinum, is a kind of marine microalgae; because it can accumulate a large amount of oil in the algae, many oil particles can be seen under the microscope, and the oil content is more than 50%, which is more than some current Oil crops such as soybeans, rapeseed, etc., and the growth cycle of these oil crops takes several months, not only limited by seasons and regions, but also takes up a lot of land; while the growth cycle of Schizochytrium is only 4-5 days, and is not affected by Restricted by seasons, regions and land, the large-scale cultivation of Schizochytrium has become an ...

AI Technical Summary

Problems solved by technology

[0002] Schizochytrium limacinum, also known as Schizochytrium limacinum, is a kind of marine microalgae; because it can accumulate a large amount of oil in the algae, many oil particles can be seen under the microscope, and the oil content is more than 50%, which is more than some current Oil crops such as soybeans, rapeseed, etc., and the growth cycle of these oil crops takes several months, not only limited by seasons and regions, but also takes up a lot of land; while the growth cycle of Schizochytrium is only 4-5 days, and is not affected by Restricted by seasons, regions and land, the large-scale cultivation of Schizochytrium has become an emerging bio-industry; Schizochytrium is not only a potential raw material for biodiesel and edible oil, but also a raw material for the health care pharmaceutical industry; The oil contains a lot of unsaturated fatty acids, especially DHA (docosahexaenoic acid), commonly known as brain gold, which has the functions of intelligence and brain, promotes brain and intellectual development of children, delays brain atrophy, and prevents Alzheimer's disease. Anticoagulation reduces the formation of thrombus, prevents myocardial infarction and cerebral infarction, lowers blood lipids and prevents arteriosclerosis, anti-inflammation, and anti-cancer effects; moreover, unsaturated fatty acids are nutrients for marine aquatic larvae, so Schizochytrium is a good marine Biological bait; however, most of the current heterotrophic cultivation techniques of Schizochytrium sp. are based on microbial fermentation process. The disinfection process requires high-temperature steam sterilization followed by forced cooling, which not only requires a large amount of coal and cooling water, but also requires a large number of additional facilities. Such as boilers, cooling towers, etc.; most of the fermentation reactors are pressure vessels, coupled with corresponding environmental protection measures, the basic construction costs and operating costs are relatively high; the present invention uses chemical methods to sterilize at room temperature, and only a small amount of disinfectant is needed per ton of culture medium and energy consumption, the entire disinfection and sterilization process reduces construction and operation costs and corresponding energy consumption, and is a low-cost, high-energy-saving disinfection process