Release method capable of accurately tracing released Chinese prawns

A Chinese prawn, precise technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of high individual mortality, demanding individual size requirements, and inability to accurately distinguish released and wild prawn individuals

Inactive Publication Date: 2012-09-26
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing detection methods cannot provide an accurate return rate due to various defects. The main reasons are as follows: 1) The released and wild prawn individuals cannot be accurately distinguished in the returned samples; 2) The physical marking process of the released individuals The operation is cumbersome and cannot be carried out on a large scale. Generally, only some released individuals are marked; 3) Physical marking has strict requirements on individual specifications, and the individual mortality after marking is high, which affects the accurate estimation of the return rate.

Method used

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  • Release method capable of accurately tracing released Chinese prawns
  • Release method capable of accurately tracing released Chinese prawns
  • Release method capable of accurately tracing released Chinese prawns

Examples

Experimental program
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Embodiment 1

[0020] Embodiment 1: Establishment of marking and releasing populations and methods

[0021] 1. Tracing individuals of Chinese shrimp families with specific molecular fingerprints

[0022] The offspring bred by each pair of parents have a consistent DNA molecular fingerprint, which is unique to the individual / family elephant and distinguishes it from other families and individuals, and lasts a lifetime. Using this specific molecular fingerprint and referring to the parental genotype, a specific individual / family can be accurately identified from a mixed population. The microsatellites of Penaeus chinensis are widely distributed in the genome, and the level of genetic variation is rich, which has the characteristics of genetic selection neutrality. The molecular fingerprint formed by the combination of different genotypes of microsatellite molecular markers is an ideal tool for individual / family identification. Taking the Chinese prawn microsatellite loci independently develo...

Embodiment 2

[0029] Example 2: Small scale testing

[0030] In 2010, a simulation experiment of releasing water by using the method of the present invention was carried out at the Aoshanwei Experimental Base of the Yellow Sea Fisheries Research Institute. The specific implementation method is: randomly collect 100 sea-caught prawns in the autumn of the first year, and physically mark the individuals through eye-stalk markers, select 6 unmated female shrimps and 6 male shrimps, and control the mating according to the ratio of 1:1 to complete the mating. Male shrimp were stored at -76°C. Also set up a backup group. The remaining female shrimps and male shrimps mate randomly, and the female shrimps that have completed the mating are mixed with the control mating females to live through the winter. In the spring of 2010, 3 controlled mating females bred families separately. In May of that year, when the individual larvae reached about 3 cm, 100 were randomly selected from each of the 3 famil...

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Abstract

The invention discloses a release method capable of accurately tracing the released Chinese prawns, belonging to the field of proliferation release of seawater animals. The method comprises the following steps of: selecting multiple parents of the Chinese prawns; performing one-to-one directional copulation on the male and female parents; sleeving a mark ring on the eyestalks of the female prawns after successful copulation, and performing overwintering cultivation; marking the eyestalks of the male prawns and placing at -70 DEG C for standby; performing offspring seed cultivation of copulated female prawns next year; releasing cultivated seedlings of the Chinese prawns in the release season; in autumn, fishing and sampling at migration points; after recapturing the samples, detecting released individuals and numbers by using a Chinese prawn fluorescence labeling microsatellite quadruple PCR individual recognition technology; and finally determining the release effect of the Chinese prawns. According to the method, the detection method of the recaptured individuals adopts the Chinese prawn fluorescence labeling microsatellite quadruple PCR individual recognition technology; the released individuals are recognized from the recaptured samples by using molecular fingerprint spectrum analysis; each mark is an individual genetic material and does not bring any internal or physical injury to the individual; and the release effect of the Chinese prawns can be accurately evaluated.

Description

technical field [0001] The invention belongs to the field of seawater animal multiplication and release, and in particular relates to a release method capable of realizing accurate traceability of individual released Penaeus sinensis. Background technique [0002] Chinese prawn (Fenneropenaeus chinensis) is a large-scale annual economic shrimp unique to my country. It is mainly distributed in the coastal areas of the Yellow Sea and Bohai Sea, and its economic benefits are remarkable. Since the 1980s, due to the influence of comprehensive factors such as increasing fishing intensity, ecological changes, water pollution, frequent disease occurrences, and artificial breeding industry's blind demand for wild Penaeus chinensis, the wild resources of Penaeus prawns in China have shrunk sharply. In order to protect and restore Chinese prawn populations and fishery resources, my country began to transplant / proliferate and release Chinese prawns in specific sea areas in 1984, releasi...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王伟继孔杰金显仕
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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