Chinese prawn releasing effect assessment method

A technology of Chinese prawns and primer pairs, applied in biochemical equipment and methods, microbial determination/inspection, climate change adaptation, etc. problem, to achieve the effect of accurate discharge effect and obvious survival/death advantage

Inactive Publication Date: 2013-06-19
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing detection methods cannot provide an accurate return rate due to various defects, mainly for the following reasons: 1) The number of released and wild prawns cannot be effectively distinguished in the returned samples; 2) The physical marking process of released individuals The operation is cumbersome and cannot be carried out on a large scale; 3) Physical marking has strict requirements on the specifications of each individual, and the individual mortality after marking is high, which affects the accurate estimation of the return rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Establishment of internal standard population, release population and evaluation method

[0018] 1. Acquisition of Specific Molecular Fingerprints of Chinese Penaeus Shrimp Family

[0019] The offspring bred by each pair of parents have a consistent DNA molecular fingerprint, which is unique to the individual / family and is different from other families and individuals and lasts a lifetime. Using this specific molecular fingerprint and referring to the parental genotype, a specific individual / family can be accurately identified from a mixed population. The microsatellites of Penaeus chinensis are widely distributed in the genome, and the level of genetic variation is rich, which has the characteristics of genetic selection neutrality. The molecular fingerprint formed by the combination of different genotypes of microsatellite molecular markers is an ideal tool for individual / family identification. Taking the Chinese prawn microsatellite loci independently de...

Embodiment 2

[0028] Example 2: Small scale testing

[0029]In 2010, a simulation experiment of Chinese prawn molecular marker release was carried out at the Aoshanwei Experimental Base of the Yellow Sea Fisheries Research Institute. In May of that year, when the individual prawns reached about 3 cm in size, two Chinese prawn sibling families were randomly selected, and 100 individuals were randomly selected from each family for fluorescent labeling, and then mixed with 1988 prawn individuals of the same size. In mid-August of that year, after all the individuals were collected, family members were identified and counted according to the information of individual fluorescent markers. Among them, 93 individuals with red fluorescent markers were detected, with a survival rate of 93%; and 81 individuals with blue fluorescent markers, with a survival rate of 81%. ; There were 1722 unmarked individuals, and the survival rate was 86.62%. After two and a half months of simulated release, the prop...

Embodiment 3

[0030] Example 3: Large-Scale Detection

[0031] In the autumn of the first year, individuals with large size, complete limbs, no mating and no obvious symptoms were selected from 200 sea-caught prawns for isolation and quarantine. The swimming appendages of prawns were taken for quantitative PCR detection of WSSV in vivo, and the individuals who did not carry WSSV virus after quarantine were paired with male and female shrimps one by one. From them, 30 mating female shrimps were selected and artificially overwintered; the mating male shrimps were completed and stored at -70°C for later use. In the spring of the following year, 3 female shrimps were selected to adopt standardized family breeding procedures, and the seedlings were cultivated as internal standard populations. Combined with the breeding and production technology of non-specific pathogenic seedlings, the seedlings of other sea-caught Penaeus sinensis populations were simultaneously cultivated separately. Each fam...

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Abstract

A Chinese prawn releasing effect assessment method includes the following steps: firstly, Chinese prawn families provided with specific molecule fingerprints are added to a release group in proportion and are released as an internal label group; secondly, Chinese prawns are recaptured at the release position as a sample, and the number of internal label individuals in the recaptured sample is determined according to the specific molecule fingerprints; and thirdly, according to the number of the internal label individuals in the second step, the number of the released group in the recaptured sample is calculated, and the releasing effect catch rate of the Chinese prawns can be finally determined. By means of the Chinese prawn releasing effect assessment method, the labeled individuals can be rapidly identified and distinguished from other individuals. The Chinese prawn releasing effect assessment method can be used for accurately estimating the number of released and wild group in the recaptured sample and the recaptured rate. Besides, the release of the labeled individuals does not destroy or affect the wild Chinese prawn group resource structure. Therefore, the Chinese prawn releasing effect assessment method can be used for accurately assessing the releasing effect of the Chinese prawns.

Description

technical field [0001] The invention belongs to the technical field of evaluating the effect of breeding and releasing seawater animals, and specifically relates to a method for accurately evaluating the effect of large-scale artificial releasing of Penaeus sinensis and the amount of wild resources efficiently, continuously and accurately. Background technique [0002] Chinese shrimp (Fenneropenaeus chinensis) is a large-scale annual economic shrimp unique to my country. It is mainly distributed in the coastal areas of the Yellow Sea and Bohai Sea, and its economic benefits are remarkable. However, since the 1980s, due to the continuous increase in fishing intensity, combined with the impact of ecological changes, water pollution, frequent disease occurrences, and blind demand for wild Penaeus chinensis in artificial breeding, the amount of wild Penaeus chinensis resources in China has been reduced. shrinking sharply. In order to protect and restore Chinese prawn population...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C12Q1/68
CPCY02A40/81
Inventor 王伟继孔杰金显仕张凯
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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