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Portunus trituberculatus release effect evaluation method

A technology of portunus trituberculatus and individuals, which is applied in the field of seawater animal release, can solve the problems of being unable to scientifically evaluate the level of supplementation of wild resources by the released population, and the inability to accurately distinguish the released individuals from the recaptured population from wild individuals and their quantity, achieving obvious survival/ Death advantage, effect of simplified preparation

Inactive Publication Date: 2017-03-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

However, due to the inability to accurately distinguish the released individuals from the wild individuals and their quantity in the recaptured population, it is impossible to scientifically assess the supplementary level of the released population to wild resources, analyze the dynamic changes of wild resources and formulate a scientific release plan. This is the urgent release of Portunus trituberculatus major issues to be addressed

Method used

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  • Portunus trituberculatus release effect evaluation method
  • Portunus trituberculatus release effect evaluation method
  • Portunus trituberculatus release effect evaluation method

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Embodiment 1: Establishment of marking and releasing populations and methods

[0054] 1. Tracing of Individuals of Portunus trituberculatus Family with Specific Molecular Fingerprints

[0055] The offspring bred by each pair of parents have a consistent DNA molecular fingerprint, which is unique to the individual / family and is different from other families and individuals and lasts a lifetime. Using this specific molecular fingerprint and referring to the parental genotype, a specific individual / family can be accurately identified from a mixed population.

[0056] The microsatellites of Portunus trituberculatus are widely distributed in the genome, and the level of genetic variation is rich, which has the characteristics of genetic selection neutrality. The molecular fingerprint formed by the combination of different genotypes of microsatellite molecular markers is an ideal tool for individual / family identification. Taking the microsatellite loci of Portunus trituberc...

Embodiment 2

[0071] Example 2: Small scale testing

[0072] In 2014, the method of the present invention was used to conduct a simulation experiment of releasing fish in Haifeng Aquaculture Co., Ltd., Changyi, Weifang City, Shandong Province. The specific implementation method is as follows: in August 2014, the research group used wild individuals of Portunus trituberculatus to construct 6 full-sib families, which were named family A to family F; Muscle samples from the female parent of the family, stored in a -80°C refrigerator; Cultivated to the second stage of juvenile crabs, 200 individuals were randomly selected from each family, and mixed with 6 families of the same specification cultivated by Haifeng Company, and 200 individuals from each family were mixed; By the time of harvest, all individuals took the large claw muscle tissue and extracted DNA; the Portunus trituberculatus SSR primers described in Table 1 were used to form a multiplex PCR reaction system for Portunus trituberculat...

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Abstract

The invention brings forward a portunus trituberculatus release effect evaluation method. The method comprises the following steps: A, on the eve of yearly release, collecting wild portunus trituberculatus captured from sea, after heating cultivation, choosing parent crabs with eggs, after baby crabs are produced, collecting female parent large crab muscle samples, and storing the samples in a refrigerator at a temperature of -80 DEG C; B, releasing young crabs when the young is cultivated to a second phase; and C, in autumn, performing capture sampling of releasing individuals at each release migration point, after the samples are recaptured, detecting release individuals and the quantity of the release individuals through a three-group fluorescent label micro satellite multiple PCR individual identification technology, and finally determining the release effect of the portunus trituberculatus. According to the invention, under the condition that a single parent is known, the re-captured portunus trituberculatus can be accurately identified, a parent cultivation process is omitted, and enormous manpower, material and financial resources are saved.

Description

technical field [0001] The invention belongs to the field of releasing seawater animals, and in particular relates to a method for evaluating the effect of releasing Portunus trituberculatus. Background technique [0002] Portunus trituberculatus (Portunus trituberculatus) is an important economic crab species in the coastal areas of China. In 2013, the aquaculture output of Portunus trituberculatus in my country reached 109,600 tons, and the aquaculture area exceeded 29,300 hectares. Because of its rapid growth and rich breeding profits, it has become an important breeding species in coastal areas of China. In recent years, with the expansion of artificial breeding scale in coastal areas, the increase of fishing intensity, artificial release and ecological environment changes, the wild germplasm resources of Portunus trituberculatus have been greatly affected. In order to protect and restore Portunus trituberculatus populations and fishery resources, my country has begun ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06Q10/06G06Q50/02A01K61/59
CPCG06Q10/0639G06Q50/02Y02A40/81
Inventor 任宪云高保全刘萍李健王俊
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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