Simplified releasing method capable of accurately tracing released Fenneropenaeus chinensis

An accurate technology for Chinese prawns, applied in the fields of climate change adaptation, animal husbandry, fish farming, etc., which can solve the problems of strict requirements on individual specifications, accurate estimation of the return catch rate, and cumbersome operation, and achieve the effect of simplifying the preparation.

Inactive Publication Date: 2013-03-20
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Application Information

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Problems solved by technology

However, the existing detection methods cannot provide an accurate return rate due to various defects. The main reasons are as follows: 1) It is impossible to accurately distinguish released and wild prawn individuals in the returned samples; 2) The physical marking process of released individuals The operation is cumbersome and cannot be carried out on a large scale. Generally, only some released individuals are marked; 3) Physical marking has strict requirements on individual specifications, and the individual mortality after marking is high, which affects the accurate estimation of the return rate.

Method used

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  • Simplified releasing method capable of accurately tracing released Fenneropenaeus chinensis
  • Simplified releasing method capable of accurately tracing released Fenneropenaeus chinensis
  • Simplified releasing method capable of accurately tracing released Fenneropenaeus chinensis

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Embodiment 1

[0027] Embodiment 1: Establishment of marking and releasing populations and methods

[0028] 1. Tracing individuals of Chinese shrimp families with specific molecular fingerprints

[0029]The offspring bred by each pair of parents have a consistent DNA molecular fingerprint, which is unique to the individual / family and is different from other families and individuals and lasts a lifetime. Using this specific molecular fingerprint and referring to the parental genotype, a specific individual / family can be accurately identified from a mixed population. The microsatellites of Penaeus chinensis are widely distributed in the genome, and the level of genetic variation is rich, which has the characteristics of genetic selection neutrality. The molecular fingerprint formed by the combination of different genotypes of microsatellite molecular markers is an ideal tool for individual / family identification. Taking the Chinese prawn microsatellite loci independently developed by the labo...

Embodiment 2

[0036] Example 2: Small scale testing

[0037] In 2010, a simulation experiment of releasing water by using the method of the present invention was carried out at the Aoshanwei Experimental Base of the Yellow Sea Fisheries Research Institute. The specific implementation method is: randomly collect 10 sea-caught mating female shrimps in spring, and physically mark the individuals through eye-stalk markers. The mating female shrimps are individually cultivated into families. 100 tails were randomly selected for physical fluorescent labeling. Randomly collected 2000 larvae of the same size and 300 individuals from the above three families for polyculture. In mid-August of that year, after all individuals were collected, the first group of Chinese shrimp fluorescently labeled microsatellite quadruple PCR reaction system described in Table 1 was used. The composition of the PCR reaction system and the amplification procedure were the same as in Example 1. Using the quadruple PCR ...

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Abstract

A simplified releasing method capable of accurately tracing released Fenneropenaeus chinensis comprises the following steps: in the releasing season each year, capturing wild mated female shrimps and tagging eyestalks of the wild mated female shrimps; then culturing seeds; preserving the female shrimps having production finished in a refrigerator at an ultralow temperature of -75 DEG C; in the autumn, capturing and sampling released individuals at each releasing migration point; detecting the released individuals and the number of the released individuals through two groups of Fenneropenaeus chinensis fluorescent labeled micro-satellite quadruple PCR (Polymerase Chain Reaction) individual identification technologies after samples are captured; and finally determining the releasing effect of the Fenneropenaeus chinensis. According to the invention, the released Fenneropenaeus chinensis can be tracked and identified as long as samples are collected from Fenneropenaeus chinensis parents producing the released seeds and parent-child traces of the collected released samples are analyzed by using the two groups of fluorescent labeled micro-satellite quadruple PCRs. Complicated operations of preparing Fenneropenaeus chinensis full-sib families on a large scale, culturing parents of the overwintering Fenneropenaeus chinensis and identifying male parent shrimps in the prior art are avoided.

Description

technical field [0001] The invention belongs to the field of releasing seawater animals, and in particular relates to a simple and easy-to-operate releasing method capable of realizing accurate traceability of released Penaeus sinensis individuals. Background technique [0002] Chinese shrimp (Fenneropenaeus chinensis) is a large-scale annual economic shrimp unique to my country. It is mainly distributed in the coastal areas of the Yellow Sea and Bohai Sea, and its economic benefits are remarkable. Since the 1980s, due to the influence of comprehensive factors such as increasing fishing intensity, ecological changes, water pollution, frequent disease occurrences, and artificial breeding industry's blind demand for wild Penaeus chinensis, the wild resources of Penaeus prawns in China have shrunk sharply. In order to protect and restore Chinese prawn populations and fishery resources, my country began to transplant / proliferate and release Chinese prawns in specific sea areas i...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12Q1/68
CPCY02A40/81
Inventor 王伟继张凯孔杰金显仕阮晓红
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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