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Cellulose sample and application thereof

A kind of technology of cellulose, microcrystalline cellulose

Active Publication Date: 2014-09-10
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The flaws in the above methods affect the accuracy of the conclusions

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  • Cellulose sample and application thereof
  • Cellulose sample and application thereof
  • Cellulose sample and application thereof

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Embodiment Construction

[0030] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

[0031] In the present embodiment, ionic liquid, [C 2 mim]OAc), purchased from Lanzhou Institute of Chemical Physics, purity ≥ 98.5%. Microcrystalline cellulose, with a degree of polymerization of 210-240, was purchased from Sinopharm Chemical Reagent Co., Ltd. The lignin was extracted and purified from ball-milled triploid Populus tomentosa by using groundwood lignin (MWL). The triploid Populus tomentosa used is 6 years old, collected from Shandong Province, and its Klason lignin content is measured as 19.9% ​​according to the TAPPI standard. Cellulase (Celluclast 1.5L) and β-glucosidase were purchased from Sigma Aldrich. All other reagents used were of analytical grade or reagent grade and were directly purchased and used without further purification.

[0032] Cellulose sample, it...

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Abstract

The invention discloses a cellulose sample and application thereof, wherein the cellulose sample is prepared by a method comprising the following steps of: a, respectively dissolving microcrystalline cellulose and mixture of the microcrystalline cellulose and different quantities of lignin into ionic liquid to obtain a lot ionic liquid solution; b, respectively transferring the ionic liquid solution into reversed solvent to regenerate and reconstruct; c, respectively filtering the regenerated and reconstructed ionic liquid solution to obtain a plurality of regenerated celluloses as cellulose samples after freezing and drying the solution. The cellulose sample disclosed by the invention can adjust single influencing factor on enzyme hydrolysis, so as to improve the accuracy of a conclusion about an experiment of affecting enzyme hydrolysis.

Description

technical field [0001] The invention relates to the technical field of lignocellulose treatment, in particular to a cellulose sample and its application. Background technique [0002] With the reduction of petroleum resources and the increasing severity of the global environmental crisis, the development of new energy sources using renewable resources as raw materials has become an inevitable choice for all countries in the world (Kunkes et al., 2008). The way of producing first-generation biofuels by fermenting or refining corn, sugarcane, and soybeans as raw materials is gradually abandoned due to obvious limitations, such as raising food prices and increasing deforestation (Fairley, 2011) . Subsequently, the preparation of second-generation biofuels from agricultural and forestry wastes, urban wastes, herbaceous plants, hardwood and coniferous wood and other non-grain renewable lignocellulose has become a research and commercial hotspot in various countries (Somma et al....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B16/00C12Q1/34
Inventor 袁同琦许凤孙润仓张利鸣孙少妮
Owner BEIJING FORESTRY UNIVERSITY