Method for extracting water-soluble carotenoid from plant materials through enzymatic hydrolysis

A plant raw material, carotene technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc.

Inactive Publication Date: 2012-10-03
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Plants such as carrots, tomatoes, red peppers, and marigolds are rich in carotenoids. Using them as raw materials to extract β-carotene, lycopene, capsa...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 100 parts of fresh carrots, add 30 parts of water, homogenize with a homogenizer for 10 minutes, and then crush with an ultrasonic crusher for 5 minutes; add 0.2 parts of pectinase, 0.2 parts of cellulase, and 0.01 part of Tween-20; the mixed liquid was enzymolyzed at 20°C and pH 3.0 for 24 hours under stirring; 2 parts of diatomaceous earth were added to the solution after enzymolysis, filtered with a filter, and the obtained filtrate Vacuum-dried to obtain water-soluble carotenoids with an extraction rate of 7.3 μg / g.

Embodiment 2

[0024] Get 100 parts of fresh carrots, add 300 parts of water, homogenize with a homogenizer for 10 minutes, then crush with an ultrasonic breaker for 5 minutes; add 8.0 parts of pectinase, 8.0 parts of cellulase, 0.2 parts of Tween-40; enzymolyze the mixed liquid at 40°C and pH6.0 for 24 hours under stirring; add 2 parts of diatomaceous earth to the solution after enzymolysis, filter with a filter, and then obtain The filtrate was vacuum-dried to obtain water-soluble carotenoids with an extraction rate of 15.6 μg / g.

Embodiment 3

[0026] Get 100 parts of fresh carrots, add 150 parts of water, homogenize with a homogenizer for 10 minutes, then crush with an ultrasonic breaker for 5 minutes; add 4.0 parts of pectinase, 4.0 parts of cellulase, 0.1 part of Tween-60; enzymolyze the mixed liquid at 50°C and pH5.5 for 24 hours under stirring; add 2 parts of diatomaceous earth to the solution after enzymolysis, filter with a filter, and then obtain The filtrate was vacuum-dried to obtain water-soluble carotenoids with an extraction rate of 12.5 μg / g.

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PUM

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Abstract

The invention relates to a method for extracting water-soluble carotenoid from carrots, tomatoes, red peppers, marigold and the like through enzymatic hydrolysis. The method comprises the following steps: adding water to fresh plant materials to obtain homogenate; carrying out ultrasonic cell disruption to the homogenate; mixing the homogenate with cellulase and pectinase and stirring the mixture while adding surfactant to the mixture; performing enzymatic hydrolysis; filtering the hydrolyzed mixture with a filter; and drying the filtrate in vacuum to obtain the water-soluble carotenoid.

Description

technical field [0001] The invention belongs to the field of food biotechnology, in particular to a method for extracting water-soluble carotenoids from plant raw materials by enzymatic hydrolysis. Background technique [0002] The color, aroma, taste and form of food are the four basic parameters to evaluate the performance quality of food, and also an important basis for consumers to choose food. In order to maintain and improve the color of food, it is often necessary to artificially color food during food processing. Food coloring is a kind of additive that makes food color and improves food color, also often called coloring agent. [0003] Food coloring can be divided into food synthetic coloring and food natural coloring according to its source and nature. Food synthetic colorings are pigments obtained by chemical synthesis, while food natural colorings are pigments obtained from natural raw materials through separation and purification. Because edible synthetic pig...

Claims

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Application Information

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IPC IPC(8): C09B61/00
Inventor 欧阳杰张玮玮杨佳李贤施显赫刘伯言武彦文李冰宁
Owner BEIJING FORESTRY UNIVERSITY
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