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Method for extracting, separating and purifying anthocyanin from blueberry peel and residues

A technology for separating and purifying anthocyanins, which is applied in the field of green chemical technology to achieve the effects of simple operation and production, improving comprehensive utilization rate and reducing production waste discharge.

Inactive Publication Date: 2012-10-10
GUIYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned extraction, separation and purification methods related to blueberry anthocyanins can prepare anthocyanin extracts with a certain purity and yield, due to the limitation of technical means, fresh blueberry fruits are often used as raw materials for preparation, and toxic organic substances are used in the processing process. Solvents or chemical preparations, or acidified organic solvents, make the product have defects such as high raw material cost, poor safety or purity, low yield, and high equipment cost, resulting in insufficient product quality and market competitiveness

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of crude blueberry anthocyanin extract: Take 0.5kg blueberry peel and residue, add 7.5L of edible ethanol with a volume concentration of 50%, filter at 45°C for 1 hour, add 5L of edible ethanol with a volume concentration of 50%, and add 5L of edible ethanol with a volume concentration of 45 °C for 1 hour and then filtered. Combine the crude filtrates from the two extractions, filter with suction, concentrate the filtrate under the conditions of -0.05~-0.08MPa, 45~50°C, recover ethanol, and dry in vacuum to obtain 9.50 g of crude anthocyanin extracts. The measured anthocyanin purity is 8.12%.

[0032]The activated AB-8 macroporous resin was selected as the resin for separation and purification of blueberry anthocyanins, and the crude extract of blueberry anthocyanins was prepared into a 0.95 mg / mL solution with distilled water. At room temperature, pass 5.0BV of the solution through a separation column equipped with AB-8 macroporous resin at a flow rate of ...

Embodiment 2

[0035] The crude extract of blueberry anthocyanins was prepared according to Example 1.

[0036] The activated AB-8 macroporous resin was used as the resin for separation and purification of blueberry anthocyanins, and the crude extract of blueberry anthocyanins was prepared into a 1.0 mg / mL solution with distilled water. At room temperature, 6.0BV of the solution was adsorbed through a separation column equipped with AB-8 macroporous resin at a flow rate of 2.5BV / h. After reaching saturation adsorption, it was washed with 5BV of distilled water at a flow rate of 2BV / h. Then use 5BV of edible ethanol with a volume concentration of 50% to elute anthocyanins at a flow rate of 2.5BV / h, collect the eluate, concentrate the eluate at -0.06MPa and 45°C, recover ethanol, and vacuum freeze-dry , 0.5662 g of purified blueberry anthocyanin extract was obtained. Using the pH differential method, the anthocyanin yield was measured to be 5.96%, the purity was 25.8%, and the anthocyanin extr...

Embodiment 3

[0039] The crude extract of blueberry anthocyanins was prepared according to Example 1.

[0040] The activated AB-8 macroporous resin was used as the resin for separation and purification of blueberry anthocyanins, and the crude extract of blueberry anthocyanins was prepared into a 1.10 mg / mL solution with distilled water. At room temperature, 4.0BV of the solution was passed through a separation column equipped with AB-8 macroporous resin at a flow rate of 3BV / h for adsorption. After saturated adsorption, it was washed with 6BV of distilled water at a flow rate of 3BV / h. Then use 6BV of edible ethanol with a volume concentration of 50% to elute the anthocyanins at a flow rate of 3BV / h, collect the eluate, concentrate the eluate at -0.08MPa and 50°C, recover the ethanol, and vacuum freeze-dry. Obtained 0.5778 grams of purified blueberry anthocyanin extract, using the pH differential method, the anthocyanin yield was 6.08%, the purity was 25.7%, and the anthocyanin extraction r...

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Abstract

The invention relates to a method for extracting, separating and purifying anthocyanin from blueberry peel and residues and belongs to the technical field of agriculture and food science. Effective ingredients are separated and extracted from fruit processing waste residues, so the method is also relevant to three-waste utilization. According to the method, edible alcohol is used for extracting anthocyanin crude extracts from blueberry peel and residues at 45 to 50 DEG C, and in addition, AB-8 macroporous resin is used for separation and purification. The method provided by the invention has the characteristics that the technology is novel, the operation and the production are simple and convenient, the low cost is low, the yield is high, the environment-friendly effect is realized; and the utilization rate of blueberry fruits is improved, the content of the anthocyanin in the separated product is greater than or equal to 26 percent, the requirements of various function food, health care food, medicine and food additives on the anthocyanin are met, and wide purposes are realized.

Description

technical field [0001] This research involves a green chemical process technology for extracting and purifying blueberry anthocyanins from blueberry peel and residue, especially a green separation and purification method using AB-8 macroporous resin to separate and purify anthocyanins in blueberry peel and residue . [0002] Background technique: [0003] Blueberry scientific name Vaccinium, belongs to Rhododendronaceae (Ericaaeae), Vacciniuml spp (Vacciniuml spp), is a perennial deciduous or evergreen shrub. Originally produced and mainly produced in the United States, blueberries have been introduced and cultivated by countries all over the world. In recent years, there has been an upsurge in blueberry planting in China. After more than ten years of planting and development, my country has formed blueberries covering the vast regions of Northeast, North, South and Southwest. In the planting area, more than 70 species of blueberry plants have been introduced, and the boom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 杜超马立志王金华刘晓燕刘志刚高蓬明王瑞
Owner GUIYANG UNIV
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