Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gene encoding alkaline beta-glucosidase and application thereof

A glucosidase and alkaline technology, applied in the fields of genetic engineering and enzyme engineering, can solve the problems of limited application range, low cellulase activity, and affecting enzymatic hydrolysis efficiency

Inactive Publication Date: 2013-09-25
GUANGXI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of various mycotoxins in the Trichoderma fermentation product, and the low activity of cellulase, especially the low activity of β-glucosidase, cellobiose accumulates in the reaction system and affects the enzymatic hydrolysis efficiency. Its scope of application is limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene encoding alkaline beta-glucosidase and application thereof
  • Gene encoding alkaline beta-glucosidase and application thereof
  • Gene encoding alkaline beta-glucosidase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0166] The following implementation methods are for better explaining the present invention, but should not be construed as limiting the present invention.

[0167] The materials used in the embodiments of the present invention include: Escherichia coli (Escherichia coli) strain XL1-blue, vector pUC19 (purchased from Dalian TaKaRa Company); expression vector pSE380 purchased from Stratagene Company, purchased from TaKaRa, MBI restrictions Sexual endonucleases, modifying enzymes and other reagents.

[0168] The present invention will be described in detail below by embodiment:

[0169] 1. Construction of Paenibacillus klebsiella GX-4 Genomic Library

[0170] Genomic DNA of Paenibacillus cookii GX-4 (Paenibacillus cookii GX-4) was extracted according to the instructions of the bacterial genomic DNA extraction kit Biospin Bacteria Genomic DNA Extraction Kit (Cat. No. BSC12S1) of BioFlux Company.

[0171] The genome library of Paenibacillus cookii GX-4 (Paenibacillus cookii GX-4...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Theoretical molecular weightaaaaaaaaaa
Login to View More

Abstract

The present invention discloses a gene encoding alkaline beta-glucosidase and application thereof. A gene Pbgl of the alkaline beta-glucosidase contains a nucleotide sequence of SEQ ID NO. 1; and an amino acid sequence of the said gene encoding the alkaline beta-glucosidase is provided, and shown as a SEQ ID NO. 2. Activity of the alkaline beta-glucosidase adapts a pH range of 5-10, and an optimum pH is 8.0. When pH is 10, enzymatic activity still retains at 58% of the highest activity, and is superior to the activity of an existing alkaline beta-glucosidase. Therefore, the alkaline beta-glucosidase has improved application value, and is suitable for application to the decomposition of cellooligosaccharide.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and enzyme engineering, and relates to a gene encoding alkaline β-glucosidase and application thereof. Background technique [0002] β-glucosidase (beta-glucosidase, EC3.2.1.21), also known as β-D-glucoside glucohydrolase, widely exists in various organisms from microorganisms to vertebrates. It can hydrolyze terminal non-reducing β-D-glucosidic bonds such as salicin, cellobiose, cellotriose, cellotetrose and p-nitrobenzene -β-D-glucoside (pNPG), etc., play an important role in the release of food flavor substances and cellulose degradation (SesteloA.B.F, Poza M., Villa T.C.β-Glucosidase activity in a Lactobacillusplantarum wine strain.World Journal of Microbiology & Biotechnology, 2004, 20: 633-637). [0003] Energy shortage is one of the major problems facing this century. As the main polysaccharide product of plant photosynthesis, cellulose is the most abundant carbohydrate on ea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/56C12N9/42C12N15/63C12N15/70C12N1/21C12P19/14C12R1/01C12R1/19
Inventor 杜丽琴霍云龙黄日波庞浩王子龙韦宇拓
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com