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Application of Geraniin extracted from Geranium sibiricum Linne in preparation of anti-herpes virus medicaments

A technology for anti-herpes virus and geranium can be used in anti-viral agents, medical preparations containing active ingredients, pharmaceutical formulas, etc., which can solve the problem of high price of western medicine, little impact on the incubation period and recurrence of herpes virus, and taking cycle. long-term problems, to achieve the effect of good anti-herpes virus activity, low toxicity and resistance to drug resistance

Inactive Publication Date: 2012-10-17
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are some anti-HSV Chinese patent medicines and Western medicines on the market. Chinese patent medicines generally have the problems of long taking cycle and ineffective curative effect. Patients need to endure the torture of the virus for a long time. Western medicines have problems such as high price and large side effects.
At present, acyclovir (ACV) and famciclovir (FCV) are the second-generation broad-spectrum antiviral drugs with large consumption in the world. It is ideal, and has little effect on the incubation period and recurrence times of herpes virus, and has side effects like neurotoxicity. Therefore, the development of low-toxicity and high-efficiency antiviral natural product drugs will have broad market prospects

Method used

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  • Application of Geraniin extracted from Geranium sibiricum Linne in preparation of anti-herpes virus medicaments
  • Application of Geraniin extracted from Geranium sibiricum Linne in preparation of anti-herpes virus medicaments
  • Application of Geraniin extracted from Geranium sibiricum Linne in preparation of anti-herpes virus medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Cytotoxicity analysis of geraniin extracted from geranium

[0023] (1) Cell culture

[0024] Vero cells were grown in DMEM monolayer medium containing 5% fetal bovine serum and 100 mg / mL penicillin and streptomycin. Cells were passaged at confluence. Cells were placed on 24-well and 6-well plates for cytotoxicity and antiviral analysis, respectively.

[0025] (2) Virus incubation

[0026] HSV-1 and HSV-2 strains were purchased from Wuhan University Virus Research Institute. When the Vero cells grow into a monolayer, the virus is inoculated and placed in CO 2 Cultivate in an incubator, and when the cytopathy reaches more than 90%, put it into a -80°C refrigerator. Combined after repeated freeze-thaw 3 times.

[0027] (3) Cytotoxicity analysis

[0028] A. Place the cells on a 96-well plate and culture at 37°C for 24 hours.

[0029] B. Remove the culture medium, add geraniin containing appropriate dilution, 8 repetitions for each concentration. 200 μL medium co...

Embodiment 2

[0052] Example 2: Analysis of anti-herpes virus activity of geraniin extracted from Geranium in animals

[0053] To test the anti-HSV effect of geraniin, 60 mice (provided by the Animal Experiment Center of Harbin Medical University, each weighing 18-22 grams) were needed for the in vivo experiment. Administration high dose group (I), middle dose group (II), low dose group (III), positive control group (IV) and blank control group (V), I, II, III, IV group mice respectively tail vein The injection titer is 0.3mL×10 -8 HSV-1, intragastric administration after fasting overnight (24 hours), group I administration 100mg / kg, group II administration 75mg / kg, group III administration 50mg / kg, group IV administration of normal saline every day, continuous After administration for seven days, the state of the mice was observed every day, the body weight was weighed, and the food was rationed. Fourteen days later, all mice were sacrificed by decapitation and all mice were dissected, a...

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Abstract

The invention relates to application of Geraniin extracted from Geranium sibiricum Linne in preparation of anti-herpes virus medicaments. The Geraniin has an obvious inhibition ability on herpes simplex virus type I (HSV-1) and herpes simplex virus type II (HSV-2) of herpesviruses. After adding different excipients, the Geraniin can be processed into any dosage form in pharmaceutics. The Geraniin is characterized by small toxic and side effect, exact curative effect, security and reliability, etc., and Geranium sibiricum Linne has abundant resources, so that the invention provides broad market prospects for developing natural product medicaments with low toxicity and high antiviral efficiency.

Description

technical field [0001] The invention relates to the application of geraniin extracted from geranium in the preparation of anti-herpes virus medicine, which belongs to the field of herbal medicine development. Background technique [0002] Geranium sibiricum Linne, also known as Geranium sibiricum Linne, is a perennial herb belonging to the Geranium sibiricum genus Geranium sibiricum. It grows on hillsides, grasslands and roadsides. Distributed in my country's Liaoning, Jilin, Heilongjiang, Hebei, Jiangsu, Anhui, Zhejiang, Hunan, Sichuan, Guizhou and Yunnan. Geranium can dispel all winds and itchy skin, cure muscle and bone pain, phlegm and fire flaccidity, spasms of hands and feet, numbness, facilitate urination, purify accumulated heat in the bladder, attack and disperse all sores and swollen toxins, relieve tuberculosis and fever, and treat wind, fire and insect teeth Pox rash scabies embolism ("Southern Yunnan Materia Medica"). Dispelling wind, dredging channels and prom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7024A61P31/22
Inventor 付玉杰祖元刚彭霄王薇赵春健
Owner NORTHEAST FORESTRY UNIVERSITY
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