Preparation technology of oral vaccine for resisting salmonella

An oral vaccine and preparation process technology, applied in the field of biotechnology and pharmacy, can solve problems such as cross-reaction, insignificant immune response effect, and no vaccine records, and achieve improved stability, stable production and preparation process, and convenient administration. Effect

Inactive Publication Date: 2012-10-17
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0022] The above-mentioned patents and papers all have solutions for the treatment and prevention of Salmonella, but they can be used for treatment as much as possible, or although they can be effe...

Method used

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  • Preparation technology of oral vaccine for resisting salmonella
  • Preparation technology of oral vaccine for resisting salmonella
  • Preparation technology of oral vaccine for resisting salmonella

Examples

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Embodiment Construction

[0056] The technical scheme of the present invention is as follows: the present invention first constructs Escherichia coli expressing Salmonella SEF14 and SEF21 pilus antigens to express SEF14 and SEF21 pilus antigens, and then extracts and purifies the SEF14 and SEF21 pilus antigens through protein purification technology. Dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylserine and cholesterol are dissolved in an organic solvent in an appropriate proportion and fully mixed to prepare mixed lipids. Dry the mixed lipids on a rotary evaporator, and then put them on a high-vacuum desiccator for further drying to form a mixed lipid film. Add a certain amount of SEF14 and SEF21 antigens to the mixed lipid film and mix thoroughly. Then, the lipid membrane is vortex dispersed under a certain intensity to obtain liposome-encapsulated SEF14 and SEF21 oral vaccines of uniform size. By centrifugation, pili antigens not encapsulated by liposomes were removed, and the antigens of li...

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Abstract

The invention discloses a preparation technology of an oral vaccine for resisting salmonella. The preparation technology comprises the following steps of constructing escherichia coli for expressing antigens of salmonella SEF14 and SEF21 fimbriaes, carrying out fimbrial antigen purification by a protein purification technology, dissolving dipalmitoyl phosphatidyl choline, dipalmitoyl phosphatidylserine and cholesterol in an organic solvent, fully mixing, drying mixed lipid, adding a certain amount of the antigens of SEF14 and SEF21 into a mixed lipid membrane, fully mixing, and carrying out lipid membrane vortex-type dispersion to obtain the oral vaccine. The preparation technology greatly improves the purity of salmonella antigens, and realizes stable, simple and controllable production having a high yield and high purity. The oral vaccine obtained by the preparation technology has effects of effectively resisting an acid environment and a damage caused by a body enzyme system. Through the preparation technology, SEF14 and SEF21 fimbriae protein antigens can be applied by an oral method. The preparation technology realizes complete detoxification and high immunogenicity and does not produce environmental pollution.

Description

technical field [0001] The invention relates to the field of biotechnology and pharmacy, more specifically, relates to the preparation of Salmonella oral vaccine. Background technique [0002] According to the findings of the Food and Agriculture Organization of the United Nations and the World Health Organization on international food safety, the incidence of food-borne diseases and food-borne diseases has been increasing in many countries in recent decades, which is related to the increase in diseases caused by microorganisms in food. related. Among the bacterial food poisoning in various countries in the world, the one caused by Salmonella often ranks first. Salmonellosis is a zoonotic disease. Poultry meat and eggs are the main vectors. It is characterized by diarrhea, fever, abdominal pain or cramps, vomiting, headache and nausea, and can lead to death in severe cases. Deaths are more common in vulnerable groups, including infants, the elderly, and those with compromi...

Claims

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Application Information

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IPC IPC(8): A61K39/112A61K47/28C12N1/21C12N15/11C12N15/31C12Q1/68A61P31/04
CPCY02A50/30
Inventor 李文哲王惠国刘庆平
Owner DALIAN UNIV
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