Branched soluble glucose polymers for peritoneal dialysis
A technology of glucose polymer and peritoneal dialysis fluid, applied in peritoneal dialysis, dialysis system, drug combination, etc.
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example 1
[0268] Example 1. Preparation of branched soluble glucose polymers of the invention (polymers according to the first family of the invention) using only branching enzymes
[0269] A starch solution containing 11% dry matter was prepared from pea starch that was enriched with a starch content greater than 95% and had an amylose content of 36.7%.
[0270] For this, 110 g of dry pea starch were resuspended in 890 ml of water at ambient temperature and with stirring.
[0271] The pH was adjusted to 7.0 with 4% NaOH.
[0272] The complete dissolution of the starch is carried out in a jet cooker at a temperature of 150 °C for a duration of 4 minutes at an air pressure of 4-5 bar, followed by atmospheric rapid cooling to 70 °C by recirculation in a heat exchanger .
[0273] The glycogen branching enzyme purified from Bacillus stearothermophilus was continuously added, and the addition ratio was per 100 g of dry substrate, 3 ml of an enzyme solution at 45000 U / ml.
[0274]The enzym...
example 2
[0293] Example 2. Preparation of branched soluble glucose polymers according to the invention (second family of polymers of the invention) using branching enzymes in combination with an alpha-amylase
[0294] A starch solution containing 11% dry matter was prepared from pea starch that was enriched with a starch content greater than 95% and had an amylose content of 36.7%.
[0295] For this, 110 g of dry pea starch were resuspended in 890 ml of water at ambient temperature and with stirring.
[0296] The pH was adjusted to 7.0 with 4% NaOH.
[0297] The complete dissolution of the starch is carried out in a jet cooker at a temperature of 150 °C for a duration of 4 minutes at an air pressure of 4-5 bar, followed by atmospheric rapid cooling to 70 °C by recirculation in a heat exchanger .
[0298] The glycogen branching enzyme purified from Bacillus stearothermophilus was continuously added, and the addition ratio was per 100 g of dry substrate, 2 ml of an enzyme solution at 4...
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