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Method for simultaneously extracting tea polysaccharide and tea polyphenol with subcritical water

A subcritical water and tea polysaccharide technology, applied in the field of deep processing of agricultural products, can solve problems such as limited solubility, and achieve the effects of short extraction time, easy operation and simple process.

Active Publication Date: 2014-03-26
湖州柳荫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Water at room temperature is a highly polar component, suitable for extracting highly polar active components, while medium and weakly polar components have limited solubility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Grinding: Take 100g of tea leaves and grind them into particles with a particle size of 0.1mm to obtain tea powder;

[0018] (2) Extraction: Put the tea powder obtained in step (1) into a stainless steel extraction kettle, and use subcritical water as the extraction liquid to perform dynamic subcritical water extraction on the tea powder, and the extraction time is 40 minutes; the subcritical water in the stainless steel extraction kettle The water pressure is 10MPa, the temperature is 120°C, and the flow rate is 6.7L / h;

[0019] (3) Adsorption and elution: take the extracted solution obtained in step (2) after cooling down at room temperature, and flow through the adsorption column chromatography equipped with adsorption resin at the same time. Three eluents;

[0020] (4) Post-treatment: Take the adsorption effluent and water eluate obtained in step (3), concentrate and dry under reduced pressure at 40-75°C to obtain a product containing tea polysaccharides and te...

Embodiment 2

[0023] (1) Grinding: Take tea leaves and grind them into particles with a particle size of 0.2 mm to obtain tea powder;

[0024] (2) Extraction: Put the tea powder obtained in step (1) into a stainless steel extraction kettle, and use subcritical water as the extraction liquid to perform dynamic subcritical water extraction on the tea powder, and the extraction time is 60 minutes; the subcritical water in the stainless steel extraction kettle The water pressure is 12MPa, the temperature is 140°C, and the flow rate is 8L / h;

[0025] (3) Adsorption and elution: take 4L of the extracted solution obtained in step (2) after room temperature cooling, and flow through the adsorption column chromatography equipped with adsorption resin at the same time, and use 1 column volume of water, 2.5 column volumes of 10% of the Wash the column with ethanol and 80% ethanol of 3 column volumes, collect the adsorption effluent and three eluents;

[0026] (4) Post-treatment: Take the adsorption e...

Embodiment 3

[0029] (1) Grinding: Take tea leaves and grind them into particles with a particle size of 0.1mm to obtain tea powder;

[0030] (2) Extraction: Put the tea powder obtained in step (1) into a stainless steel extraction kettle, and use subcritical water as the extraction liquid to perform dynamic subcritical water extraction on the tea powder. The extraction time is 80 minutes; the subcritical water in the stainless steel extraction kettle The water pressure is 10MPa, the temperature is 160°C, and the flow rate is 12L / h;

[0031] (3) Adsorption and elution: Take 5L of the extracted solution obtained in step (2) after room temperature cooling, and flow through the adsorption column chromatography equipped with adsorption resin at the same time, sequentially use 1 column volume of water, 2 column volumes of 10% of the Wash the column with ethanol and 2.5 column volumes of 80% ethanol, collect the adsorption effluent and three eluents;

[0032] (4) Post-treatment: take the adsorpt...

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Abstract

The invention provides a method for simultaneously extracting tea polysaccharide and tea polyphenol with subcritical water, belonging to the technical field of deep processing of agricultural products. The method comprises the steps of adding tea powder to an extracting kettle, carrying out dynamic extraction with subcritical water in certain condition, simultaneously carrying out macroporous resin column chromatography on the cooled extract for dynamic adsorption, and respectively eluting the adsorption column with ethanol solution of different concentrations after extraction and adsorption are finished. According to the invention, tea polyphenol and tea polysaccharide are simultaneously extracted, the extraction time is short, the extraction efficiency is high, toxic organic solvent is not used in the extraction separation process, and environment-friendly effect is achieved; and extraction and separation are coupled, and the process is simple, easy to operate and low in production cost.

Description

technical field [0001] The invention discloses a method for simultaneously extracting tea polysaccharides and tea polyphenols with subcritical water, belonging to the technical field of deep processing of agricultural products. Background technique [0002] Tea polysaccharides and tea polyphenols are the two main functional components of tea, which have broad-spectrum biological activities. Among them, tea polyphenols have anti-cancer, anti-virus, anti-bacterial, anti-radiation, lipid-lowering, blood pressure-lowering, anti-caries, deodorizing, etc. Function; tea polysaccharides can lower blood sugar and blood fat, and also have obvious effects in preventing and treating diabetes, anticoagulation, anti-thrombosis, protecting blood phase and enhancing human non-specific immunity. The two are widely used in food, medicine, health care and other fields It has broad development and application prospects. [0003] The extraction methods of tea polyphenols mainly include reflux, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07G99/00C07D311/62
Inventor 王洪新马朝阳娄在祥尔朝娟唐弟康谭吉良兰海崇
Owner 湖州柳荫生物科技有限公司