Gene and protein of grass carp interleukin 10 and recombinant expression method thereof

Abstract

The present invention relates to a gene and a protein of grass carp interleukin 10 and a recombinant expression method thereof. The present invention provides a gene sequence of grass carp interleukin 10 and a grass carp interleukin 10 protein sequence coded by the gene sequence, and puts forward a prokaryotic recombinant expression method of the grass carp interleukin 10. A homologous cloning technology is employed to obtain a cDNA complete sequence of the grass carp interleukin 10 gene; a mature peptide sequence of the grass carp interleukin 10 gene is employed to construct prokaryotic expression vectors; and screening is carried out to obtain a prokaryotic expression system for efficient expression of the grass carp interleukin 10 gene. The method comprises specific steps of: 1. gene cloning; 2. construction of the recombinant expression vector; and 3. expression of the recombinant protein, and separation and purification of expression products. The invention has beneficial effect that the technical scheme of the invention can realize industrialized production of the recombinant grass carp interleukin 10, thereby applying the recombinant grass carp interleukin 10 to theoretical study on the mechanism of immunity of fish.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a technology for recombinant expression and purification of grass carp interleukin 10 gene. Background technique [0002] Interleukin-10 (IL-10) is an important cytokine. In 1989, Fiorentino et al. discovered that mouse Th2 cells could secrete a substance that inhibits Th1 cells from secreting interferon and other cytokines, so they named this substance cytokine synthesis inhibiting factor (CSIF), and later called white blood cells. Interleukin 10. Subsequent studies have found that this cytokine plays an important role in inflammation, malignant tumors, and autoimmune diseases. It can inhibit the proliferation and differentiation of T cells and macrophages, and can also promote the stable differentiation of B cells. Its immune diversity makes it play an important role in the immune system. Current research also shows that interleukin 10 has immunomodulatory function a...

AI Technical Summary

Problems solved by technology

So far, the prevention of grass carp and aquatic animal diseases mainly relies on drugs, but drugs are often used to prevent and control diseases, and pathogens are likely to develop resistance to certain drugs, resulting in failure of control. The ecological balance is destroyed, and the use of immunological methods to prevent and treat diseases can not only avoid this disadvantage, but also improve product quality

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