Use of synthetic polysulphated oligosaccharides as cleaning agents for a wound
A technology of polysulfated oligosaccharides and detergents, which is applied in the direction of active carbohydrate ingredients, medical preparations containing active ingredients, drug combinations, etc., and can solve the problems of slow action and insufficient removal of a large amount of fibrin tissue
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Embodiment 1
[0105] Example 1: Demonstration of the effect of sucrose octasulfate potassium salt degradation in degrading fibrin matrices prepared in vitro
[0106] 1. Preparation of Fibrin Matrix in Vitro
[0107] Fibrin matrices were prepared according to Brown's experimental notes described in "Fibroblast migration in fibrin gel matrices" (Am J. Pathol, 1993, 142: 273-283).
[0108] The components and procedures used are as follows:
[0109] Dissolve the following substances at 37°C:
[0110] - 5 ml of an aqueous solution containing 50 mmoles of Hepes (Sigma-Aldrich Catalog);
[0111] - 15 mg of human plasma fibrinogen (Sigma-Aldrich Catalog);
[0112] -5 mmol CaCl 2 .
[0113] To the solution thus prepared was added 50 µl of thrombin, 100 NIH of human plasma (Sigma-Aldrich Catalog).
[0114] The reaction mixture was mixed, added to 15 ml tubes (or 60 mm diameter Petri dishes), and incubated at 37°C.
[0115] During the first 24 h of incubation at 37°C, the appearance of a rough,...
Embodiment 2
[0142] Example 2: Demonstration of the effect of sucrose octasulfate potassium salt on fibrin tissue taken from the body
[0143] The same solution of potassium sucrose octasulfate was tested on fibrinous tissue removed from the body.
[0144] For this test, a 5mm 2 Fibrin tissue samples were placed in 15ml tubes incubated at 37°C.
[0145] After 24 h, 1 ml of sucrose octasulfate potassium salt solution was deposited on the samples.
[0146] Prepare negative control with PBS again, use Prepare a positive control.
[0147] PDFs were determined 48h after the introduction of the active substance or the control.
[0148] This experiment was repeated on 5 fibrin tissue samples obtained from the same patient.
[0149] Thus, it was found that sucrose octasulfate potassium salt completely degrades the fibrin matrix in this test, demonstrating its effectiveness.
[0150] This result was confirmed by the determination of the PDFs as follows:
[0151] Higher than 20μg / ml;
[0...
Embodiment 3
[0155] Example 3: Demonstration of the effectiveness of sucrose octasulfate potassium salt incorporated into absorbent dressings in degrading fibrin matrices prepared in vitro
[0156] 1. Preparation of Absorbent Dressings Containing Potassium Sucrose Octasulfate
[0157] Using thermal bonding technology, with the help of TOYOBOCOLTD company sold F superabsorbent fibers and bicomponent thermally bonded polyester / polyethylene fibers (ratio of 70% superabsorbent fibers / 30% thermally bonded fibers), prepared to be used as absorbent dressings in case of autolytic cleaning 180g / m used 2 non-woven fabric.
[0158] Separately, a hydrocolloid viscous mass comprising sucrose octasulfate potassium salt was prepared by mixing in a MEL G-40 mixer.
[0159] The composition of this viscous mass is expressed in weight percent relative to the total weight of the viscous mass as follows:
[0160] - by the Shell company to Mineral oil sold under the name 917: 32.8%;
[0161] -CMC by AQU...
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