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Molecular marking method for detecting imidazolone herbicide resisting gene of cabbage type rape

A Brassica napus anti-imidazolinone technology, applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., can solve the problems of high cost and cumbersome operation

Active Publication Date: 2012-11-07
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0008] The object of the present invention is to utilize DNA sequencing technique to detect resistance gene BnALS1R The resulting disadvantages are cumbersome operation and high cost. According to the imidazolinone herbicide resistance gene of Brassica napus BnALS1R A single nucleotide difference with the DNA sequence of the ALS gene, and a molecular marker linked to it is obtained, and a simple method is used to quickly and accurately detect the resistance gene BnALS1R , and further marker-assisted selection

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  • Molecular marking method for detecting imidazolone herbicide resisting gene of cabbage type rape
  • Molecular marking method for detecting imidazolone herbicide resisting gene of cabbage type rape
  • Molecular marking method for detecting imidazolone herbicide resisting gene of cabbage type rape

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Embodiment Construction

[0049] All methods in the following implementation are conventional methods unless otherwise specified, and the specific implementation steps are as follows:

[0050] (1) Test materials

[0051] Conventional rape varieties Ningyou No. 12, Ningyou No. 14, Ningyou No. 16, Ningyou No. 18, and Ningyou No. 20 are approved varieties in Jiangsu Province; MICMS double-low restorer line 3075R (Pu Huiming et al., 2002, Jiangsu Agricultural Science , 4:33-34); imidazolinone herbicide-resistant mutant M9 (Hu Maolong et al., Imidazolinone herbicide-resistant Brassica napus mutant gene and its application; Chinese patent ZL201010232607.4) and offspring selection line M9 -2, M9-3, M9-11, M9-14 (Pu Huiming et al., Inheritance and application of imidazolinone resistance traits in rape. Chinese Journal of Oil Crops, 33(1): 15-19)

[0052] BnALS1R Genotype segregation population: F2 population (3075R / M9-2), 3075R is the female parent, M9-2 is the male parent crossing and selfing; BC1 populatio...

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Abstract

The invention provides a three-primer molecular marking method for detecting an imidazolone herbicide resisting gene of cabbage type rape and belongs to the field of plant molecular breeding. Primer pairs of AP15F / AP18R and AP15F / AP19R are added into two polymerase chain reaction (PCR) reaction systems respectively to subject cabbage type rape deoxyribonucleic acid (DNA) to amplification, and homozygote free of resistance gene BnALS1R, homozygote containing resistance gene BnALS1R and heterozygote containing resistance gene BnALS1R are distinguished. By means of the method, whether rapeseed germplasm resources or breeding population of the rape contain the imidazolone herbicide resisting gene BnALS1R or not can be rapidly and accurately detected, whether the contained herbicide gene is the homozygote or the heterozygote can be accurately distinguished, the selection efficiency of the resistance gene BnALS1R can be improved, and the breeding process of imidazolone herbicide resisting rape breeds can be accelerated.

Description

[0001] technical field [0002] The invention relates to the field of plant molecular breeding, in particular to a method for detecting genes resistant to imidazolinone herbicides in Brassica napus BnALS1R molecular labeling methods. Background technique [0003] Weeds in farmland seriously affect the yield and quality of rapeseed, and have become one of the main biological disasters in rapeseed production. The popularization and application of herbicide-resistant rapeseed is an important way to overcome field weed damage. Compared with traditional rapeseed, herbicide-resistant rapeseed has three advantages: first, the farming operation is simple and efficient, and growers only need to spray herbicides once or twice at the seedling stage to effectively kill field weeds; Second, it can greatly reduce the cost of rapeseed production and increase profits; third, it can promote less-tillage and no-tillage, which is conducive to water and soil conservation and energy saving. Ho...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 胡茂龙浦惠明戚存扣张洁夫高建芹龙卫华陈松付三雄陈锋周晓婴张维
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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