MicroRNA (micro ribonucleic acid) for regulating gene expression of B7-H3 (a molecule of the B7 family)

A B7-H3 and gene expression technology, applied in the field of microRNA to regulate B7-H3 gene expression, can solve the problems of different functions, difficult miRNAs, and no correlation between miR-143 and B7-H3 genes.

Inactive Publication Date: 2012-11-14
SUZHOU UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006]Although it is known in the art that certain microRNAs are related to tumors, there are many kinds of miRNAs known in the art with different functions. It is difficult to screen out specific miRNAs that are related to tumors and can be used as pathogenesis, treatment options and prognosis
At present, there is no report on the correlation between miR-143 and B7-H3 gene in this field

Method used

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  • MicroRNA (micro ribonucleic acid) for regulating gene expression of B7-H3 (a molecule of the B7 family)
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  • MicroRNA (micro ribonucleic acid) for regulating gene expression of B7-H3 (a molecule of the B7 family)

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Embodiment Construction

[0026] The above solution will be further described below in conjunction with specific embodiments. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

[0027] 1. Reagents and materials

[0028] 1. Reagents

[0029] Fetal bovine serum (Hyclone, USA); complete medium: add 100ml of fetal bovine serum, 0.15g of L-glutamine, 10.0ml of 2-mercaptoethanol (5×10 -3 mol / L); liposome 2000 (Invitrogen, USA); penicillin, streptomycin (Shanghai Sangon Biotechnology Co., Ltd); TRIzol (Invitrogen, USA); agarose (LP0028A, Oxoid Ltd, UK); gel Electrophoresis sample solution and ethidium bromide (EtBr) (Shanghai Sangon Biotechnology Co., Ltd.); gel recovery DNA kit an...

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Abstract

The invention discloses microRNA (micro ribonucleic acid) for regulating gene expression of B7-H3 (a molecule of the B7 family). The microRNA is nucleic acid, functional segment or variant including a sequence of 5'-UGAGAUGAAGCACUGUAGCUC-3'. The microRNA is miR-143. Performed in-vitro biological function tests show that hsa-miR-143 is capable of inhibiting molecular expression of the B7-H3 by bonding with 3'-UTR (untranslated regions) of the B7-H3 gene, and antitumor effect is achieved by regulating a signal path of the B7-H3 and / or expression and functions of the miR-143.

Description

Technical field [0001] The present invention is a field of biotechnology and medical technology. Specifically, the invention involves the field of gene expression regulation and control technology. It specially involves a small RNA used to regulate the B7-H3 gene expression. Background technique [0002] The tumor has always threatened human health and life, and the traditional treatment methods are not satisfactory.Immunotherapy can induce the body to produce specific anti -tumor immune response by increasing the immuneness of tumors, and to achieve the purpose of treating tumors. It is a passive and active biological treatment method.Cell -mediated cellular immunity is the main form of anti -tumor immunity. The body activates T cells through specific identification of tumor antigen to produce corresponding immune killing effects. [0003] Industry has confirmed that the effective activation and function of T cells require the synergy of dual signals: first, antigen peptide-MHC ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113
Inventor 汪维鹏朱健洁张学光
Owner SUZHOU UNIV
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