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Process method for producing ethanol by utilizing low-temperature cellulase to perform simultaneous saccharification and fermentation

A technology of simultaneous saccharification fermentation and low-temperature cellulase, which is applied in the fields of bioengineering and energy, can solve the problem that normal temperature enzymes cannot reach the highest enzymatic degradation efficiency, achieve good application and market requirements, improve efficiency, and reduce costs.

Inactive Publication Date: 2012-11-14
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve this technical problem, we use low-temperature cellulase (the optimum enzyme activity is around 38°C) for synchronous saccharification and fermentation, which can meet the fermentation temperature of alcoholic yeast with the optimum enzyme activity, and solve the problem that the use of normal temperature enzymes cannot achieve the highest enzyme activity. The problem of degradation efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The process method is to add the produced low-temperature cellulase and alcoholic yeast into the fermenter at the same time, and use the raw material rich in cellulose as the substrate to carry out simultaneous saccharification and fermentation. The steps are as follows:

[0016] ①. Add the low-temperature cellulase-producing strain into the fermenter for fermentation;

[0017] 2. When the enzyme activity of the above-mentioned fermentation liquid reaches 4U, harvest the fermentation liquid;

[0018] ③. Add initial cellulose raw materials, low-temperature cellulase fermentation broth, and alcoholic yeast to the above-mentioned fermentation broth to carry out simultaneous saccharification and fermentation.

[0019] ④. When the concentration of ethanol in the simultaneous saccharification fermentation broth reaches 4%, carry out distillation at 80°C for 30 minutes to obtain cellulosic ethanol with a content of 97%.

[0020] The cellulose raw material is Suaeda salsa, the...

Embodiment 2

[0022] The process method is to add the produced low-temperature cellulase and alcoholic yeast into the fermenter at the same time, and use the raw material rich in cellulose as the substrate to carry out simultaneous saccharification and fermentation. The steps are as follows:

[0023] ①. Add the low-temperature cellulase-producing strain into the fermenter for fermentation;

[0024] 2. When the enzyme activity of the above-mentioned fermentation liquid reaches 4U, harvest the fermentation liquid;

[0025] ③, adding initial cellulose raw materials, low-temperature cellulase fermentation liquid, and alcoholic yeast to the above fermentation liquid to carry out simultaneous saccharification and fermentation;

[0026] ④. When the concentration of ethanol in the simultaneous saccharification fermentation broth reaches 6%, carry out distillation at 80°C for 30 minutes to obtain cellulosic ethanol with a content of 98%.

[0027] The cellulose raw material is Suaeda salsa, the soli...

Embodiment 3

[0029] The process method is to add the produced low-temperature cellulase and alcoholic yeast into the fermenter at the same time, and use the raw material rich in cellulose as the substrate to carry out simultaneous saccharification and fermentation. The steps are as follows:

[0030] ①. Add the low-temperature cellulase-producing strain into the fermenter for fermentation;

[0031] 2. When the enzyme activity of the above-mentioned fermentation liquid reaches 4U, harvest the fermentation liquid;

[0032] ③. Add initial cellulose raw materials, low-temperature cellulase fermentation broth, and alcoholic yeast to the above-mentioned fermentation broth to carry out simultaneous saccharification and fermentation.

[0033] ④. When the ethanol concentration in the simultaneous saccharification fermentation broth reaches 10%, carry out distillation at 80° C. for 30 minutes to obtain cellulose ethanol with a content of 99%.

[0034] The cellulose raw material is Suaeda salsa, the ...

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Abstract

The invention provides a process method for producing ethanol by utilizing low-temperature cellulase to perform simultaneous saccharification and fermentation. The process method is characterized by simultaneously adding low-temperature cellulase and alcohol yeast for production into a fermentation tank and taking a raw material which is rich in the cellulase as a substrate to perform the simultaneous saccharification and fermentation, and the process method comprises the following steps: (1) simultaneously adding the low-temperature cellulase and the alcohol yeast into the fermentation tank and fermenting for 3-5 days; (2) harvesting a fermentation solution; (3) adding the initial cellulase raw material, a low-temperature cellulase fermentation solution and the alcohol yeast into the fermentation solution and performing the simultaneous saccharification and fermentation; and (4) getting cellulosic ethanol. The process method disclosed by the invention has the effects of improving efficiency and reducing cost, and when the produced cellulase is applied to the bench-scale-level simultaneous saccharification and fermentation, the biological ethanol with higher quality can be produced, so that application and market requirements can be well met.

Description

technical field [0001] The invention relates to a process for producing ethanol, in particular to a process for producing ethanol by synchronous saccharification and fermentation using low-temperature cellulase, which improves efficiency and reduces costs, and belongs to the fields of bioengineering and energy. Background technique [0002] Facing the dwindling oil resources, the ever-increasing energy crisis and the resulting environmental pollution, the research on the production of fuel ethanol by fermentation of biomass as raw material has attracted more and more attention from all countries, and has become one of the key research and promotion energy topics in the world. . The rational use of biofuels can reduce the consumption of non-renewable energy, reduce air pollution and reduce greenhouse gas emissions. Therefore, to a certain extent, it can play a positive role in the rational use of natural resources, ensuring national energy security and preventing climate chan...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCY02E50/16Y02E50/10
Inventor 王能飞臧家业沈继红刘羽
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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