Unlock instant, AI-driven research and patent intelligence for your innovation.

Manufacturing method and application of PDMS (Polydimethylsiloxane) polymer chip with arc sunk holes

A polymer and polymer solution technology, applied in biochemical equipment and methods, combinatorial chemistry, library creation, etc., can solve the problems of high price and complicated operation, and achieve the effect of simple operation and low experimental cost

Active Publication Date: 2013-12-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method and application for making a PDMS polymer chip with arc-shaped concave holes, so as to solve the problems of complicated operation and high price in the previous manufacturing process.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Manufacturing method and application of PDMS (Polydimethylsiloxane) polymer chip with arc sunk holes
  • Manufacturing method and application of PDMS (Polydimethylsiloxane) polymer chip with arc sunk holes
  • Manufacturing method and application of PDMS (Polydimethylsiloxane) polymer chip with arc sunk holes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The PDMS channel template is made by soft etching technology, the template is a 12×12 circular hole, and the template structure is cylindrical. Prepare an uncured PDMS (10:1) solution, pour a certain amount of PDMS solution on a clean silicon wafer at a speed of 5,000 rpm, uniform the glue on a homogenizer for one minute, and then heat and cure at 80°C for 2 hours. Using plasma technology to seal PDMS templates and thin films, glass sheets, forming such as figure 1 installation. After the whole device was silanized for 5 minutes, connect the air cylinder, control the amount of gas entering by adjusting the air pressure, pour the uncured PDMS (10:1), feed the gas, and heat at 80°C for 20 minutes at the same time. After the device is cooled, a PDMS chip is placed on the glass to form an arc-shaped PDMS polymer chip with concave holes.

Embodiment 2

[0036] The principle and flow chart of the three-dimensional cell culture on the PDMS polymer chip with arc-shaped concave holes are as follows figure 2 shown. The PDMS polymer chip with arc-shaped concave holes is first sterilized, irradiated with ultraviolet light overnight, and then modified with 1% PF-127 solution for 8 hours, inoculated with cell suspension, and the cells will precipitate under the action of gravity To the bottom of the small wells on the surface of the chip, three-dimensional cell clusters are spontaneously formed.

Embodiment 3

[0038] Characterization of the height of a PDMS polymer chip based on pneumatically fabricated arcuate recessed apertures. image 3 The height of the arc-shaped concave holes on the surface of the PDMS polymer chip is shown under different gas pressure conditions. and image 3 A in a shows the height difference of the small holes on the surface of the PDMS polymer chip under the same air pressure conditions at different positions, which shows that arc-shaped concave small holes of different heights can be simultaneously produced under the same control conditions by this method. image 3The c in the figure shows that different heights of small holes can be formed under different air pressure conditions, which shows that arc-shaped concave small holes with different heights can be significantly controlled by this method.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a manufacturing method and application of a PDMS (Polydimethylsiloxane) polymer chip with arc sunk holes. The method comprises the following steps of: manufacturing a PDMS channel chip template by utilizing a soft etching technology; manufacturing a PDMS thin film, sealing the PDMS channel chip template and sheet glass after sealing the PDMS channel chip template and the PDMS thin film, connecting the bottom of the PDMS channel chip template with gas by a metal pipe and carrying out surface modification on an integral device; and pouring uncured PDMS polymer solution into the device, filling the gas into the device, heating and curing the PDMS polymer solution and stripping the PDMS chip. The PDMS chip subjected to surface modification can be used for three-dimensional culture of cells. The method does not require expensive etching equipment and has the advantages that the method is simple and rapid to operate, is low in experiment cost, does not relate to an organic reagent, is environment-friendly and can be integrated with other technologies.

Description

technical field [0001] The invention relates to the processing, manufacture, modification and application fields of polymer chips, and particularly provides a method and application of a PDMS polymer chip with arc-shaped concave holes produced in a pneumatic manner. Background technique [0002] Tissue engineering is a new discipline emerging in recent years. It is the application of the principles and technologies of life science and engineering, on the basis of a correct understanding of the relationship between the structure and function of mammals in both normal and pathological states, research and development for the repair, maintenance, and promotion of various tissues or organs of the human body Functional and morphological biosurrogates after injury. The core of tissue engineering is to establish a three-dimensional complex of cells and biomaterials, that is, a living tissue with vitality, which is used to reconstruct the shape, structure and function of the diseas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C40B50/14C12M3/00
Inventor 秦建华石杨
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI