Ceriporia lacerata strain and application thereof

A technology for tearing cereus bacteria and strains, which is applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., and can solve the problems of no reports

Inactive Publication Date: 2014-04-16
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the preparation of DMC is mainly obtained by extracting organic matter from the plant water wenghua, and there is no report on the preparation of DMC by fermentation of endophytic fungi in water wenghua

Method used

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  • Ceriporia lacerata strain and application thereof
  • Ceriporia lacerata strain and application thereof
  • Ceriporia lacerata strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Screening of strain Ceriopria lacerate DMC1106 (CCTCC NO: M2012201)

[0039] (1) Collect complete waterweed flower buds in a sealed bag (with a little water in the bag), store them in an incubator at 4°C, and return them to the laboratory for immediate processing.

[0040] (2) Wash the flower buds with tap water for 30 minutes, and then wash them with sterile water for 3 times.

[0041] (3) Put the preliminary treated flower buds in a sterile ultra-clean bench, carry out disinfection according to the following steps, carry out surface disinfection with 75% alcohol, rinse the tissue with sterile water for 3-4 times, and disinfect with 10% NaClO for 10 minutes. Rinse the tissue with bacterial water 3-4 times.

[0042] (4) the above-mentioned processed flower buds are cut into 0.5cm with a scalpel sterilized in advance2 Place them on the PDA medium with 200 mg / L penicillin, and cultivate them at 28±1°C. In order to check the disinfection effect, a control group experimen...

Embodiment 2

[0046] Microscopic morphology and molecular identification of strain Ceriopria lacerate DMC1106 (CCTCC NO: M2012201):

[0047] The microscopic morphology observation adopts the solid plate culture method: culture on PDA medium, culture at 28±1°C for 3 days, the colony diameter is 2-3cm, and the colony covers the entire petri dish after 6-7 days. The colony edge is uneven and radial. It is thicker in the middle, thinner at the edge, with white fluff on the surface, and the entire colony is filamentous and intertwined, which is not easy to pick. After maturity, the whole plate shows a thicker layer of membranous culture, and the back is light yellow. See figure 1 .

[0048] Molecular identification using ITS-DNA identification: take the 7-day-cultivated fermentation liquid and the pretreated bacterial liquid, centrifuge at 5000g for 5 minutes, discard the supernatant, add 300 μL of Chelex lysate to the precipitate, add 10 μL of proteinase K (invitrogen), and heat at 56°C , 1-2h...

Embodiment 3

[0050] A method for preparing 2', 4'-dihydroxy-6'-methoxy-3', 5'-dimethylchalcone (DMC) from the fermentation strain Ceriopria lacerate DMC1106 (CCTCC NO: M2012201), comprising Follow the steps below:

[0051] (1) Under sterile conditions, place the strain Ceriopria lacerate DMC1106 (CCTCC NO: M2012201) on a sterilized solid potato sucrose medium plate (PDA), and activate it at 28±1°C for 4 to 5 days;

[0052] (2) Under sterile conditions, use an inoculation loop to pick 2 to 3 loops of the strain activated in step (1) (CCTCC NO: M2012201), insert it into the sterilized improved Sabouraud liquid seed medium, and incubate at 28± Cultivate at 1°C, 150rpm for 3 days, and use as first-grade seeds;

[0053] Insert 15mL of grade 1 seeds into 200mL of sterilized modified Sabouraud liquid seed medium (glucose 20g / L, peptone 10g / L), and culture at 28±1℃, 150rpm for 2 days, and the obtained culture is used as fermentation culture the secondary seed of

[0054] (3) Insert 20mL of seco...

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Abstract

The invention relates to a ceriporia lacerata strain and an application thereof. The strain is ceriporia lacerata DMC1106, and is preserved in the China Center for Type Culture Collection on June 8th, 2012 with the preservation number CCTCC No: M2012201. By fermenting the strain (CCTCC No: M2012201) 2',4'-dihydroxy-6'-methoxyl-3',5'-dimethylchalcone (DMC) can be prepared. A novel means is provided for the preparation of DMC.

Description

technical field [0001] The invention relates to a bacterial strain of Ceriopria lacerata and its application. Background technique [0002] 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (abbreviated as DMC, its structure is shown in formula I) belongs to chalcone compounds, its It has a strong inhibitory effect on a variety of cancer cells (it can induce cancer cell apoptosis by down-regulating the transcription of bcl-2 and c-myc genes and enhancing the enzyme activity of caspase-3). Moreover, it can also significantly reverse the multidrug resistance of tumor cells by down-regulating the transcription of MRP1 and GST-π genes. It is a natural anti-tumor compound that works through multiple targets. In addition, DMC also has liver protection. , hypoglycemic and other significant effects, the market prospect is broad. [0003] [0004] In the past ten years, the production of target products by microbial fermentation has become a mature applied discipline. It has the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/26C12R1/645
Inventor 卢艳花汪杰
Owner EAST CHINA UNIV OF SCI & TECH
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