Treatment Of Cancer Or Tumor Induced By The Release Of Heat Generated By Various Chains Of Magnetosomes Extracted From Magnetotactic Bacteria And Submitted To An Alternative Magnetic Field

A technology of magnetotactic bacteria and alternating magnetic field, applied in the field of thermal therapy

Active Publication Date: 2012-12-05
NANOBACTERIE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After all, bacterial magnetosomes have not been validated fo

Method used

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  • Treatment Of Cancer Or Tumor Induced By The Release Of Heat Generated By Various Chains Of Magnetosomes Extracted From Magnetotactic Bacteria And Submitted To An Alternative Magnetic Field
  • Treatment Of Cancer Or Tumor Induced By The Release Of Heat Generated By Various Chains Of Magnetosomes Extracted From Magnetotactic Bacteria And Submitted To An Alternative Magnetic Field
  • Treatment Of Cancer Or Tumor Induced By The Release Of Heat Generated By Various Chains Of Magnetosomes Extracted From Magnetotactic Bacteria And Submitted To An Alternative Magnetic Field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0163] Preparation of different types of particles used as heat source:

[0164] In this example, we describe the method by which different types of particles are prepared for use as heat sources. These particles are the particles contained in the whole magnetotactic bacteria, the chains of magnetosomes were extracted from the magnetotactic bacteria, the individual magnetosomes were extracted from the magnetotactic bacteria and detached from the chains by heating and SDS treatment, and the magnetosomes were covered with citrate ions. Chemically synthesized superparamagnetic iron oxide nanoparticles (SPIONCitrate) or commercially available chemically synthesized nanoparticles covered with PEG molecules (SPIONPEG). SPIONPEG was purchased from the German company Micromod (product name: -D-spio, product number: 79-00-201).

[0165] SPIONCitrate is used as standard nanoparticles because they possess a similar size to most nanoparticles used for magnetic hyperthermia (see for exa...

Embodiment 2

[0175] Heat production by exposure of bacterial magnetosomes to an oscillating magnetic field

[0176] In this example, we provide a detailed study of the mechanism of heat generation of magnetosomes biomineralized by magnetotactic bacteria. The values ​​of magnetic field frequency (108kHz) and magnetic field amplitude (23 to 88mT) used to heat different samples were within the range of magnetic field parameters used for high-frequency high-amplitude AMF (alternating magnetic field) superheating (Ivkov et al., Clin. Cancer Res., 2005, 11, 7093s-7103s; De Nardo et al., Clin. Cancer Res., 2005, 11, 7087s-7092s; De Nardo et al., The J. Nucl. Med., 2007, 48, 437-444). For AMF overheating, the recommended magnetic field frequency is between 50 kHz and 1 MHz, while the magnetic field amplitude needs to be kept below 100 mT (Mornet et al., J. Mater. Chem., 2004, 14, 2161-2175). We compared the thermogenic properties of three different types of magnetosome arrangements (Alphandéry et a...

Embodiment 3

[0195] Improved heating efficiency of chains of extracted magnetosomes obtained by synthesizing magnetotactic bacteria in the presence of various chelating agents and / or transition metals.

[0196]In this example, we describe various methods for improving the heating efficiency of chains of extracted magnetosomes suspended in water. These methods use various additives introduced into the growth medium of AMB-1 magnetotactic bacteria. These additives are chelating agents such as bisphosphonate molecules, dopamine, rhodamine, EDTA or transition metals such as cobalt.

[0197] Materials and methods:

[0198] First, a growth medium for magnetotactic bacteria was prepared by following the same method as described in Example 1. Then one of the following additives was added to the growth medium of magnetotactic bacteria: 0.4 μM, 4 μM or 40 μM of different types of bisphosphonates (alendronate, risedronate or neridronate), 4 μM , 20 μM or 400 μM rhodamine solution, 0.4 μM or 4 μM E...

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Abstract

In this disclosure, we describe a method for the treatment of tumor(s) or tumor cell(s) or cancer(s) in a subject in need by the generation of heat. The latter is produced by chains of magnetosomes extracted from whole magnetotactic bacteria and subjected to an alternative magnetic field. These chains of magnetosomes yield efficient antitumoral activity whereas magnetosomes unbound from the chains or kept within the whole bacteria produce poor or no antitumoral activity. The introduction of various chemicals such as chelating agents and/or transition metals within the growth medium of the bacteria improves the heating properties of the chains of magnetosomes. Moreover, the insertion of the chains of magnetosomes within a lipid vesicle is also suggested in order to favor their rotation in vivo and hence to improve their heating capacity. The vesicle can contain an antitumoral agent together with the chains of magnetosomes. In this case, the agent is released within the tumors by heating the vesicle.

Description

technical field [0001] The present invention relates to in vivo thermal treatment of cells or tissues, especially tumors or tumor cells, using heat generated in situ by subjecting magnetic elements to an alternating magnetic field. The invention particularly relates to thermal therapy using hyperthermia or thermoablation. The type of magnetic element described in this disclosure is a chain of iron oxide nanoparticles synthesized by biological methods. Background technique [0002] The present invention describes a form of hyperthermia that can be used to destroy cancer, tumors, or tumor cells. Heat is generated by chains of bacterial magnetosomes, which are extracted from magnetotactic bacteria. Chains of magnetosomes used in hyperthermia can be obtained by culturing bacteria under various conditions as follows: [0003] (1) Magnetotactic bacteria (such as ATCC 700274) are cultured in a standard growth medium (such as ATCC medium 1653, or a growth medium similar to ATCC m...

Claims

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Application Information

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IPC IPC(8): A61K35/74
CPCC12N1/20A61K35/74C12N1/38A61K41/0052A61K33/26A61P35/00A61N1/406A61N2/00A61K9/0019A61K9/10A61K9/51A61K9/5146
Inventor E·阿尔方德里S·富尔I·舍比
Owner NANOBACTERIE
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