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Chemokine-derived peptide, its expression gene and application thereof

A technology for expressing genes and derivatizing peptides, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve problems such as antigenicity, large molecular weight, and poor stability.

Inactive Publication Date: 2012-12-12
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, a variety of natural chemokines (chemerins) and their enzyme-cleaved products found in the body are all proteins, which have disadvantages such as relatively large molecular weight, difficult preparation, antigenicity, and poor stability. It is difficult to mass-produce and develop large-scale Animal and human experimental research and drug development

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  • Chemokine-derived peptide, its expression gene and application thereof
  • Chemokine-derived peptide, its expression gene and application thereof
  • Chemokine-derived peptide, its expression gene and application thereof

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Embodiment Construction

[0068] In recent years, in order to study the active peptides of chemokines, many peptides derived from the carboxy-terminal region of chemokine precursors have been artificially synthesized, and the shortest bioactive peptides of chemokines are found to be human Chem 149-157 Also known as human chemokine C9 peptide (chemerin9), this 9-peptide has strong chemotactic activity (Wittamer et al.2004); in mice, the 15 amino acid chemokine C15 peptide (Chem 140-154 ), has anti-inflammatory effects (Cash et al.2008). This shows that the polypeptides derived from chemokines are active. This discovery reminds us that the discovery of natural chemokine polypeptides that exist and are active in the body obviously provides a form with low cost, simple process and better stability for the application of chemokines, and provides a new way for the development of related new drugs. convenient and effective way.

[0069] Chem 149-157 and Chem in mice 140-154 , although they all bind to CMK...

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Abstract

A chemokine-derived peptide is a polypeptide having the following structural formula: Xaal-Xaa2-Xaa3-Ala-Gly-Glu-Asp-Xaa4-Xaa5-Xaa6-Xaa7-Xaa8-Xaa9-Pro-Gly-Gln-Phe-Ala-Phe-Xaa10, wherein Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, Xaa6, Xaa7, Xaa8, Xaa9 or Xaa10 is any one of 20 L-amino acids. As the above chemokine-derived peptide is a polypeptide from the perspective of its chemical nature, the defect that a traditional natural chemokine and its enzyme-digested product have relatively large molecular weight and poor antigenicity and stability and are difficult to prepare is overcome. Large-batch production and development of large animal and human body experimental research and drug development can be carried out. The invention also provides an expression gene of the above chemokine-derived peptide and points out an application of the expression gene in the treatment of cancer, obesity, fatty liver, diabetes, cardiovascular disease, reproduction and metabolic syndrome.

Description

【Technical field】 [0001] The invention relates to the field of polypeptide medicine, in particular to a chemokine-derived peptide, its expression gene and its application. 【Background technique】 [0002] Protein is the most important class of biomacromolecules in the body, and is currently widely used as medicine for the treatment of diseases (Li Wei 2004). However, protein drugs also have disadvantages, such as large molecular weight, difficult preparation, antigenicity, and easy degradation in vivo. Fortunately, it has been found that some polypeptides with smaller molecular weights also have protein-like activities, and their functions are more significant. These biologically active polypeptides have very little (or even no) content in the body, but their effects are extremely strong. The occurrence and development of many diseases are related to their imbalance. The prospect of drug development is very good. Therefore, the research and development of polypeptide drugs h...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/19A61K38/10A61P35/00A61P3/04A61P1/16A61P3/10A61P9/00A61P15/00A61P3/00
Inventor 张键任培根张旭黄晨余春红马萍
Owner SHENZHEN INST OF ADVANCED TECH